Rabbit EPS15 Polyclonal Antibody | anti-EPS15 antibody

EPS15 (Phospho-Tyr849) Antibody

Cat. Num. AAA9406781

• Gene Names: EPS15; AF1P; AF-1P; MLLT5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: EPS15; Polyclonal Antibody; EPS15 (Phospho-Tyr849) Antibody; AF1P; MLLT5; Protein Eps15; anti-EPS15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of EPS15 only when phosphorylated at tyrosine 849.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 582

• Applicable Applications for anti-EPS15 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 849 (S-A-Y(p)-P-S) derived from Human Ephrin EPS15.
• Modification: Phospho-Tyr849
• Target Name: EPS15
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from JK cells (Lane 2), using EPS15 (Phospho-Tyr849) Antibody. The lane on the left is treated with antigen-specific peptide.)

Related Product Information for anti-EPS15 antibody

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.Wong W.T., Oncogene 9:1591-1597(1994).Bernard O.A., Oncogene 9:1039-1045(1994).

Product Categories/Family for anti-EPS15 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 229577185
• NCBI GeneID: 2060
• NCBI Accession #: NP_001153441.1
• NCBI GenBank Nucleotide #: NM_001159969.1
• UniProt Accession #: P42566; Q5SRH4; B2R8J7; D3DPJ2
• Molecular Weight: 64,376 Da
• NCBI Official Full Name: epidermal growth factor receptor substrate 15 isoform B
• NCBI Official Synonym Full Names: epidermal growth factor receptor pathway substrate 15
• NCBI Official Symbol: EPS15
• NCBI Official Synonym Symbols: AF1P; AF-1P; MLLT5
• NCBI Protein Information: epidermal growth factor receptor substrate 15
• UniProt Protein Name: Epidermal growth factor receptor substrate 15
• Protein Family: Epidermal growth factor receptor substrate
• UniProt Gene Name: EPS15
• UniProt Synonym Gene Names: AF1P; Protein Eps15
• UniProt Entry Name: EPS15_HUMAN

NCBI Description

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009]

Uniprot Description

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.