Rabbit anti-Mouse EphB6 Polyclonal Antibody | anti-EphB6 antibody

Anti-EphB6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112339

• Gene Names: Ephb6; Mep; Cekl
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: EphB6; Polyclonal Antibody; Anti-EphB6 Antibody; Rabbit Polyclonal; EphB6/Eph Receptor B6 Antibody; Rabbit PAb; Antigen Affinity Purified; EPH receptor B6; Anti-Cekl Antibody; Anti-Mep Antibody; anti-EphB6 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse EphB6/Eph Receptor B6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EphB6 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: Recombinant Mouse EphB6/Eph Receptor B6 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse EphB6/Eph Receptor B6 (rM EphB6/Eph Receptor B6). EphB6/Eph Receptor B6 specific IgG was purified by Mouse EphB6/Eph Receptor B6 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse EPHB6 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-EphB6 antibody

Ephrins are divided into the ephrin-A (EFNA) class and the ephrin-B (EFNB) class based on their structures and sequence relationships. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 is an unusual Eph receptor, lacking catalytic capacity due to alterations in its kinase domain. Interestingly, increased metastatic activity is associated with reduced EphB6 receptor expression in several tumor types, including breast cancer. This emphasizes the potential of EphB6 to act as a suppressor of cancer aggressiveness. EphB6 suppress cancer invasiveness through c-Cbl-dependent signaling, morphologic changes, and cell attachment and indicate that EphB6 may represent a useful prognostic marker and a promising target for therapeutic approaches. EphB6 can both positively and negatively regulate cell adhesion and migration, and suggest that tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition. In addition, Ephrin-B2 may be a physiological ligand for the EphB6 receptor.

Product Categories/Family for anti-EphB6 antibody

Actin Dynamics Signaling Pathway

References

Munthe E, et al. (2000)Ephrin-B2 is a candidate ligand for the Eph receptor, EphB6. FEBS Lett. 466(1): 169-74.Matsuoka H, et al. (2005) Biphasic functions of the kinase-defective Ephb6 receptor in cell adhesion and migration. J Biol Chem. 280(32): 29355-63.Truitt L, et al. (2010) The EphB6 receptor cooperates with c-Cbl to regulate the behavior of breast cancer cells. Cancer Res. 70(3): 1141-53.

NCBI and Uniprot Product Information

• NCBI GI #: 226437671
• NCBI GeneID: 13848
• NCBI Accession #: NP_001139823.1
• NCBI GenBank Nucleotide #: NM_001146351.1
• UniProt Accession #: O08644; Q3TQ77; Q8BN76; Q8K0A9
• Molecular Weight: 110,107 Da
• NCBI Official Full Name: ephrin type-B receptor 6
• NCBI Official Synonym Full Names: Eph receptor B6
• NCBI Official Symbol: Ephb6
• NCBI Official Synonym Symbols: Mep; Cekl
• NCBI Protein Information: ephrin type-B receptor 6; ephrin type A receptor; chicken eph/elk receptor-like protein; tyrosine-protein kinase-defective receptor EPH-6
• UniProt Protein Name: Ephrin type-B receptor 6
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb6
• UniProt Synonym Gene Names: Cekl
• UniProt Entry Name: EPHB6_MOUSE

Uniprot Description

Function: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2

By similarity.

Subunit structure: Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner

By similarity.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted.

Tissue specificity: High level in thymus, and brain. Very low levels of expression in kidney, lung, liver, bone marrow, skeletal muscle, spleen from 2 week old and adult mice, heart, testes and embryonic stem cells. Ref.1

Domain: The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2

By similarity.

Post-translational modification: Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.