Rabbit anti-Human, Mouse MYB Polyclonal Antibody | anti-MYB antibody

MYB (Transcriptional Activator Myb, Proto-oncogene c-Myb) (PE)

Cat. Num. AAA6488741

• Gene Names: MYB; efg; Cmyb; c-myb; c-myb_CDS
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Immunoaffinity Chromatography.
• Synonyms: MYB; Polyclonal Antibody; MYB (Transcriptional Activator Myb; Proto-oncogene c-Myb) (PE); anti-MYB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MYB. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A and Immunoaffinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 659

• Applicable Applications for anti-MYB antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: IHC-P: 1:50-1:100; FLISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide PLLKKIKQEVESPTCA containing a predicted sumoylation site from the C-terminal region of human MYB (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Myb Antibody (Sumo) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Myb antibody detected the Myb protein (arrow).)

Product Categories/Family for anti-MYB antibody

Antibodies; Transcription Factors

References

1. Luchetti, M.M., et al., J. Biol. Chem. 278(3): 1533-1541 (2003). 2. Dahle, O., et al., Eur. J. Biochem. 270(6): 1338-1348 (2003). 3. Chen, J., et al., Oncogene 21(12): 1859-1869 (2002). 4. Hernandez-Munain, C., et al., J. Immunol. 169(8): 4362-4369 (2002). 5. Tanno, B., et al., J. Biol. Chem. 277(26): 23, 172-23, 180 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 45502015
• NCBI GeneID: 4602
• UniProt Accession #: P10242
• Molecular Weight: 72.341
• NCBI Official Full Name: v-myb myeloblastosis viral oncogene homologue (avian)
• NCBI Official Synonym Full Names: MYB proto-oncogene, transcription factor
• NCBI Official Symbol: MYB
• NCBI Official Synonym Symbols: efg; Cmyb; c-myb; c-myb_CDS
• NCBI Protein Information: transcriptional activator Myb
• UniProt Protein Name: Transcriptional activator Myb
• Protein Family: Myb protein
• UniProt Gene Name: MYB
• UniProt Entry Name: MYB_HUMAN

NCBI Description

This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: nuclear matrix; cytosol

Molecular Function: protein binding; chromatin binding

Biological Process: positive regulation of histone H3-K9 methylation; thymus development; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; homeostasis of number of cells; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin remodeling; positive regulation of T-helper cell differentiation; regulation of transcription, DNA-dependent; calcium ion transport; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; blood coagulation; embryonic gut development; G1/S transition of mitotic cell cycle