OSMR elisa pair set

OSMR Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118363

• Synonyms: OSMR; OSMR Matched ELISA Antibody Pair Set; Human; Human OSMR/IL-31RB ELISA Pair Set; oncostatin M receptor; IL-31RB Matched ELISA Antibody Pair Set; OSMRB Matched ELISA Antibody Pair Set; PLCA1 Matched ELISA Antibody Pair Set; OSMR elisa pair set

• Specificity: Quantitative determination of Human OSMR

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 234.38-15000pg/mL
• Sensitivity: 234.4pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-OSMR monoclonal antibody, Dilute to a working concentration of 1ug/mL in CBS before coating. ; 2. Detection Antibody 0.5 mg/mL mouse anti-OSMR monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 600 ng of recombinant OSMR. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15, 000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for OSMR elisa pair set

Oncostatin-M specific receptor subunit beta also known as the oncostatin M receptor (OSMR) and Interleukin-31 receptor subunit beta (IL-31RB), is one of the receptor proteins for oncostatin M. OSMR is a member of the type I cytokine receptor family. IL-31RB/OSMR heterodimerizes with interleukin 6 signal transducer to form the type II oncostatin M receptor and with interleukin 31 receptor A to form the interleukin 31 receptor, and thus transduces oncostatin M and interleukin 31 induced signaling events. Mutations in IL-31RB/OSMR have been associated with familial primary localized cutaneous amyloidosis. Defects in IL-31RB/OSMR are the cause of amyloidosis primary localized cutaneous type 1 (PLCA1), also known as familial lichen amyloidosis of familial cutaneous lichen amyloidosis. PLCA1 is hereditary primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening (lichenification) that may be exacerbated by chronic scratching and rubbing. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins.

This OSMR Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human OSMR. It contains Human OSMR capture antibody, Human OSMR detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human OSMR protein.

References

Arita K, et al.. (2008) Oncostatin M Receptor-beta Mutations Underlie Familial Primary Localized Cutaneous Amyloidosis. Am J Hum. Genet. 82 (1): 73-80.Malaval L, et al.. (2005) GP130/OSMR is the only LIF/IL-6 family receptor complex to promote osteoblast differentiation of calvaria progenitors. J Cell Physiol. 204(2): 585-93.Lin MW, et al.. (2010) Novel IL31RA gene mutation and ancestral OSMR mutant allele in familial primary cutaneous amyloidosis. Eur J Hum Genet. 18(1): 26-32.