Sheep Dyrk (Downs Syndrome critical region) Polyclonal Antibody | anti-DYRK1A antibody

Dyrk (Downs Syndrome critical region)

Cat. Num. AAA395867

• Gene Names: DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Dyrk (Downs Syndrome critical region); Polyclonal Antibody; Dual Specificity (Tyrosine and serine/threonine kinase) Kinase; anti-DYRK1A antibody

• Host: Sheep
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 763

• Applicable Applications for anti-DYRK1A antibody: Western Blot (WB), Immunoprecipitation (IP)
• Immunogen: Recombinant protein corresponding to amino acids 486 to 763 of the mouse Dyrk1A protein.
• Positive Control: Expressed in a variety of embryonic and adult tissues. Expressed abundantly in neurons of the brain, spinal cord, and retina in developing embryos.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Testing Data

(Immunoprecipitation of)

Related Product Information for anti-DYRK1A antibody

Dyrk, the vertebrate of Drosophilia minibrain, is a dual-specificity kinase predominately expressed in the central nervous system. The human clone has been isolated from the Downs' syndrome critical region and it is potentially implicated in the neuropathology of the disease. Main features of the protein include an N-terminal nuclear translocation signal, a putative leucine zipper domain, a core kinase domain with some similarity to kinases involved in cell cycle regulation and a C-terminal PEST sequence. The Dyrk kinase can be phosphorylated on tyrosine residues, leading to an active kinase that can phosphorylated itself or exogenous substrates on both tyrosine and serine/threonine residues. Dyrk can also multimerize and translocate to the nucleus. Present studies on Dyrk suggest a potential role for this kinase in the exit from the cell cycle and the beginning of neuronal differentiation.

Product Categories/Family for anti-DYRK1A antibody

Polyclonal Antibody; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 18765758
• NCBI GeneID: 1859
• NCBI Accession #: NP_001387.2
• NCBI GenBank Nucleotide #: NM_001396.3
• UniProt Accession #: Q13627; O60769; Q92582; Q92810; Q9UNM5
• Molecular Weight: 85,584 Da
• NCBI Official Full Name: dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1
• NCBI Official Synonym Full Names: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
• NCBI Official Symbol: DYRK1A
• NCBI Official Synonym Symbols: MNB; DYRK; HP86; MNBH; MRD7; DYRK1
• NCBI Protein Information: dual specificity tyrosine-phosphorylation-regulated kinase 1A; hMNB; MNB/DYRK protein kinase; serine/threonine kinase MNB; mnb protein kinase homolog hp86; protein kinase minibrain homolog; dual specificity YAK1-related kinase; serine/threonine-specific protein kinase
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK1A
• UniProt Synonym Gene Names: DYRK; MNB; MNBH; MNBH; hMNB
• UniProt Entry Name: DYR1A_HUMAN

NCBI Description

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Phosphorylates serine, threonine and tyrosine residues in its sequence and in exogenous substrates such as CRY2, FOXO1 and SIRT1. Ref.3

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Inhibited by RANBP9. Ref.7

Subunit structure: Interacts RAD54L2/ARIP4. Interacts with CRY2

By similarity. Interacts with RANBP9. Interacts with WDR68. Ref.7 Ref.8

Subcellular location: Nucleus speckle.

Tissue specificity: Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney. Ref.1 Ref.2 Ref.3 Ref.5

Developmental stage: Expressed in the developing central nervous system. Overexpressed 1.5-fold in fetal Down syndrome brain. Ref.3

Domain: The polyhistidine repeats act as targeting signals to nuclear speckles (Ref.11).

Post-translational modification: Autophosphorylated on tyrosine residues.

Involvement in disease: Mental retardation, autosomal dominant 7 (MRD7) [MIM:614104]: A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.Contains 1 protein kinase domain.