Rabbit PACAP-27 ELISA Kit | PACAP-27 elisa kit
Rabbit PACAP-27 (Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PACAP-27. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PACAP-27 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PACAP-27, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PACAP-27. You can calculate the concentration of PACAP-27 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2010]
Uniprot Description
ADCYAP1R1: This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. Belongs to the G-protein coupled receptor 2 family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 2; Receptor, GPCR
Chromosomal Location of Human Ortholog: 7p14
Cellular Component: neuron projection; tight junction; cell surface; rough endoplasmic reticulum; integral to plasma membrane; plasma membrane; caveola; endosome; receptor complex
Molecular Function: neuropeptide binding; ADP-ribosylation factor binding; vasoactive intestinal polypeptide receptor activity; protein binding; receptor activity; adenylate cyclase binding
Biological Process: response to drug; development of primary female sexual characteristics; nerve growth factor receptor signaling pathway; adenylate cyclase activation; response to estradiol stimulus; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; cell surface receptor linked signal transduction; response to ethanol; phospholipase C activation; spermatogenesis; cell differentiation; multicellular organismal response to stress; transmembrane receptor protein tyrosine kinase signaling pathway
