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Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using ENTPD2 antibody. Blue: DAPI for nuclear staining.)

Rabbit anti-Human ENTPD2 Polyclonal Antibody | anti-ENTPD2 antibody

ENTPD2 Polyclonal Antibody

Gene Names
ENTPD2; CD39L1; NTPDase-2
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ENTPD2; Polyclonal Antibody; ENTPD2 Polyclonal Antibody; Ectonucleoside triphosphate; diphosphohydrolase 2; NTPDase 2; CD39 antigen-like 1; Ecto-ATP diphosphohydrolase 2; Ecto-ATPDase 2; Ecto-ATPase 2; CD39L1; anti-ENTPD2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ENTPD2 polyclonal antibody detects endogenous levels of ENTPD2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
495
Applicable Applications for anti-ENTPD2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
Immunogen
Recombinant full length Human ENTPD2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using ENTPD2 antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using ENTPD2 antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-ENTPD2 antibody
CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENP1), is an integral membrane glycoprotein that acts as an extracellular nucleotide-hydrolyzing enzyme. CD39 inhibits ADP-induced platelet aggregation by hydrolyzing ADP to AMP and ultimately generating adenosine. Intracellular CD39 undergoes glycosylation at 6 N-glycosylation sites and translocates to the membrane in order to be an active enzyme. CD39L1 (CD39 antigen-like 1), also known as ENTPD2 (Ectonucleoside triphosphate diphosphohydrolase 2), is a 495 amino acid multi-pass membrane protein that requires calcium and magnesium cofactors to hydrolyze ATP and other nucleotides in the regulation of purigenic neurotransmission. CD39L1 is expressed in kidney, colon, heart, testis, pancreas, brain, prostate, skeletal muscle, small intestine and ovaries. There are two isoforms of CD39L1 that are produced as a result of alternative splicing events.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
954
UniProt Accession #
Molecular Weight
~ 54kDa
NCBI Official Full Name
Ectonucleoside triphosphate diphosphohydrolase 2
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 2
NCBI Official Symbol
ENTPD2
NCBI Official Synonym Symbols
CD39L1; NTPDase-2
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 2
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 2
UniProt Gene Name
ENTPD2
UniProt Synonym Gene Names
CD39L1; NTPDase 2; Ecto-ATPDase 2; Ecto-ATPase 2
UniProt Entry Name
ENTP2_HUMAN

NCBI Description

The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

ENTPD2: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent. The order of activity with different substrates is ATP > GTP > CTP = ITP > UTP >> ADP = UDP. Belongs to the GDA1/CD39 NTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.1.-; Nucleotide Metabolism - purine; Extracellular matrix; Membrane protein, integral; Membrane protein, multi-pass; Hydrolase

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane; basal lamina

Molecular Function: nucleoside-diphosphatase activity; nucleoside-triphosphatase activity; ATP binding

Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; purine ribonucleoside diphosphate catabolic process

Research Articles on ENTPD2

Similar Products

Product Notes

The ENTPD2 entpd2 (Catalog #AAA3011607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENTPD2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENTPD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the ENTPD2 entpd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENTPD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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