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SDS-PAGE (Figure annotation denotes ug of protein loaded and % gel used.)

14.3.3 beta recombinant protein

14.3.3 beta protein

Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Applications
SDS-Page
Purity
> 90% pure
Synonyms
14.3.3 beta; 14.3.3 beta protein; Protein kinase C inhibitor protein 1 protein; Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide.; Brain protein 14 3 3 beta isoform protein; HS1 protein; 14-3-3 beta; 14-3-3 beta protein; beta polypeptide protein; KCIP-1 protein; GW 128 protein; Protein 1054 protein; YWHAB protein; HS 1 protein; 14/3/3 beta protein; beta polypeptide 14 3 3 protein beta/alpha protein; GW128 protein; KCIP 1 protein; Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein protein; KCIP1 protein; 14/3/3 beta; 14.3.3 beta recombinant protein
Ordering
For Research Use Only!
Host
Human
Purity/Purification
> 90% pure
Form/Format
Supplied as a liquid in 20mM Tris, pH 8.0, containing 1mM EDTA, and 50mM NaCl.
Concentration
1 mg/ml (varies by lot)
Sequence
1-246 amino acids: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
Sequence Length
246
Applicable Applications for 14.3.3 beta recombinant protein
SDS-PAGE
Protein Type
Recombinant
Biological Significance
The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human 14-3-3 beta was expressed in E Coli and purified by using conventional chromatography techniques.
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Figure annotation denotes ug of protein loaded and % gel used.)

SDS-PAGE (Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for 14.3.3 beta recombinant protein
Purified recombinant Human 14.3.3 beta protein
Product Categories/Family for 14.3.3 beta recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28 kDa (246 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
14-3-3 protein beta/alpha
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
NCBI Official Symbol
YWHAB
NCBI Official Synonym Symbols
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
NCBI Protein Information
14-3-3 protein beta/alpha
UniProt Protein Name
14-3-3 protein beta/alpha
UniProt Gene Name
YWHAB
UniProt Synonym Gene Names
KCIP-1
UniProt Entry Name
1433B_HUMAN

NCBI Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: cytoplasmic vesicle membrane; focal adhesion; membrane; transcriptional repressor complex; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of catalytic activity; negative regulation of protein amino acid dephosphorylation; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; protein heterooligomerization; apoptosis; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; cytoplasmic sequestering of protein; insulin receptor signaling pathway; innate immune response; gene expression; negative regulation of transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway; protein targeting

Research Articles on 14.3.3 beta

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Product Notes

The 14.3.3 beta ywhab (Catalog #AAA536781) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 14.3.3 beta can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the 14.3.3 beta ywhab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 1-246 amino acids: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN. It is sometimes possible for the material contained within the vial of "14.3.3 beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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