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Testing Data (Gel: 10%SDS-PAGE Lysate: 30 ug 293T cell lysate Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Rabbit anti-Human ENPP7 Polyclonal Antibody | anti-ENPP7 antibody

ENPP7 Antibody

Gene Names
ENPP7; NPP7; NPP-7; E-NPP 7; ALK-SMase
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Synonyms
ENPP7; Polyclonal Antibody; ENPP7 Antibody; ectonucleotide pyrophosphatase/phosphodiesterase 7; ALK-SMase; anti-ENPP7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total ENPP7 protein.
Form/Format
Supplied at 1.4mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
Sequence Length
458
Applicable Applications for anti-ENPP7 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:1000-1:5000
Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:50-1:200
Immunogen Type
Peptide-KLH
Immunogen Description
Synthetic peptide corresponding to a region derived from 290-305 amino acids of Human ectonucleotide pyrophosphatase/phosphodiesterase 7
Target Name
ENPP7
Preparation and Storage
Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGE Lysate: 30 ug 293T cell lysate Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Testing Data (Gel: 10%SDS-PAGE Lysate: 30 ug 293T cell lysate Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 31192(ENPP7 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.)

Immunohistochemistry (IHC) (The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 31192(ENPP7 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.)
Related Product Information for anti-ENPP7 antibody
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (E-NPP 7) also known as alkaline sphingomyelin phosphodiesterase (Alk-SMase) or intestinal alkaline sphingomyelinase is an enzyme that in humans is encoded by the ENPP7 gene. Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Not inhibited by ADP, AMP and EDTA. Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.
Product Categories/Family for anti-ENPP7 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,494 Da
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 7
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 7
NCBI Official Symbol
ENPP7
NCBI Official Synonym Symbols
NPP7; NPP-7; E-NPP 7; ALK-SMase
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 7
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
UniProt Gene Name
ENPP7
UniProt Synonym Gene Names
E-NPP 7; NPP-7; Alk-SMase
UniProt Entry Name
ENPP7_HUMAN

Uniprot Description

ENPP7: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: Lipid Metabolism - sphingolipid; Cell cycle regulation; DNA replication; Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.1.4.12; Phosphodiesterase

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: Golgi apparatus; microvillus; membrane; integral to membrane; plasma membrane

Molecular Function: sphingomyelin phosphodiesterase activity

Biological Process: negative regulation of cell proliferation; sphingomyelin metabolic process; sphingolipid metabolic process; negative regulation of DNA replication; glycosphingolipid metabolic process; sphingomyelin catabolic process

Research Articles on ENPP7

Similar Products

Product Notes

The ENPP7 enpp7 (Catalog #AAA9407136) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENPP7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPP7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA: 1:1000-1:5000 Western blotting: 1:1000-1:5000 Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the ENPP7 enpp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPP7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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