Western Blot (WB)
(SPTLC2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SPTLC2 antibody detected the SPTLC2 protein (arrow).)
Rabbit anti-Human SPTLC2 Polyclonal Antibody | anti-SPTLC2 antibody
SPTLC2, ID (SPTLC2, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, Long chain base biosynthesis protein 2a, Serine-palmitoyl-CoA transferase 2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(SPTLC2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SPTLC2 antibody detected the SPTLC2 protein (arrow).)
Western Blot (WB)
(SPTLC2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SPTLC2 antibody detected the SPTLC2 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. [provided by RefSeq, Mar 2011]
Uniprot Description
Function: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate. Ref.8 Ref.9
Catalytic activity: Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2. Ref.8
Cofactor: Pyridoxal phosphate
By similarity.
Pathway: Lipid metabolism; sphingolipid metabolism.
Subunit structure: Heterodimer with SPTLC1. Component of the serine palmitoyltransferase (SPT) complex, composed of LCB1/SPTLC1, LCB2 (SPTLC2 or SPTLC3) and ssPT (SPTSSA and SPTSSB). Ref.8
Subcellular location: Endoplasmic reticulum membrane; Single-pass membrane protein
By similarity.
Tissue specificity: Widely expressed. Ref.7
Involvement in disease: Hereditary sensory and autonomic neuropathy 1C (HSAN1C) [MIM:613640]: A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by prominent sensory abnormalities with a variable degree of motor and autonomic dysfunction. The neurological phenotype is often complicated by severe infections, osteomyelitis, and amputations. HSAN1C symptoms include loss of touch and vibration in the feet, dysesthesia and severe panmodal sensory loss in the upper and lower limbs, distal lower limb sensory loss with ulceration and osteomyelitis, and distal muscle weakness.Note: The disease is caused by mutations affecting the gene represented in this entry. SPTLC2 disease mutations cause a shift in the substrate specificity of SPT resulting in the alternative use of L-alanine and L-glycine over its canonical substrate L-serine. This leads to the production of 1-deoxysphingolipids that cannot be correctly metabolized (Ref.11). Ref.9 Ref.11
Sequence similarities: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
Sequence caution: The sequence BAA25452.2 differs from that shown. Reason: Erroneous initiation.
Research Articles on SPTLC2
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Product Notes
The SPTLC2 sptlc2 (Catalog #AAA646093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPTLC2, ID (SPTLC2, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, Long chain base biosynthesis protein 2a, Serine-palmitoyl-CoA transferase 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPTLC2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the SPTLC2 sptlc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPTLC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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