Mouse Glucose-6-phosphate 1-dehydrogenase X ELISA Kit | G6pdx elisa kit

Mouse Glucose-6-phosphate 1-dehydrogenase X ELISA Kit

Cat. Num. AAA288222

• Gene Names: G6pdx; G28A; G6pd; Gpdx
• Reactivity: Mouse
• Synonyms: Glucose-6-phosphate 1-dehydrogenase X; Mouse Glucose-6-phosphate 1-dehydrogenase X ELISA Kit; G6pdx elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse G6PD. No significant cross-reactivity or interference was observed.
• Sequence Length: 515

• Assay Type: Sandwich
• Detection Range: 0.78-50 mIU/mL
• Sensitivity: 0.39 mIU/mL
• Intra-Assay CV: <=6.7%
• Inter-Assay CV: <=8.9%
• Recovery: 101%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for G6pdx elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to G6PD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for G6PD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G6PD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of G6PD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6996917
• NCBI GeneID: 14381
• NCBI Accession #: NP_032088.1
• NCBI GenBank Nucleotide #: NM_008062.2
• UniProt Accession #: Q00612
• Molecular Weight: 59,263 Da
• NCBI Official Full Name: glucose-6-phosphate 1-dehydrogenase X
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase X-linked
• NCBI Official Symbol: G6pdx
• NCBI Official Synonym Symbols: G28A; G6pd; Gpdx
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase X
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase X
• Protein Family: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6pdx
• UniProt Synonym Gene Names: G6pd; G6pd-1; G6PD
• UniProt Entry Name: G6PD1_MOUSE

Uniprot Description

G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Oxidoreductase; EC 1.1.1.49; Other Amino Acids Metabolism - glutathione; Cell development/differentiation

Cellular Component: centrosome; cytoplasm; cytosol; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; microtubule organizing center; nucleus

Molecular Function: carbohydrate binding; glucose binding; glucose-6-phosphate dehydrogenase activity; identical protein binding; NADP binding; oxidoreductase activity; protein homodimerization activity

Biological Process: angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; angiotensin mediated vasodilation involved in regulation of systemic arterial blood pressure; carbohydrate metabolic process; cholesterol biosynthetic process; cytokine production; erythrocyte development; erythrocyte maturation; glucose 6-phosphate metabolic process; glucose metabolic process; glutathione metabolic process; interleukin-10 production; interleukin-12 production; NADP biosynthetic process; NADP metabolic process; NADPH regeneration; pentose biosynthetic process; pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch; regulation of multicellular organism growth; regulation of neuron apoptosis; response to ethanol; response to oxidative stress; ribose phosphate biosynthetic process