Immunofluorescence (IF)
(Immunofluorescence staining of Human ENPP7 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ENPP7 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.)
Mouse anti-Human ENPP7 Monoclonal Antibody | anti-ENPP7 antibody
Anti-ENPP7 Antibody, Mouse Monoclonal
Gene Names
ENPP7; MGC50179; ALK-SMase
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A
Synonyms
ENPP7; Monoclonal Antibody; Anti-ENPP7 Antibody; Mouse Monoclonal; ENPP7 Antibody; Mouse MAb; ectonucleotide pyrophosphatase/phosphodiesterase 7; Anti-ALK-SMase Antibody; Anti-E-NPP7 Antibody; Anti-NPP-7 Antibody; Anti-NPP7 Antibody; anti-ENPP7 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b
Clone Number
10
Specificity
Human ENPP7
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ENPP7 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:20-1:100
Immunogen
Recombinant Human ENPP7 protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ENPP7 (rh ENPP7). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of Human ENPP7 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ENPP7 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human ENPP7 in HT29 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ENPP7 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.)
Related Product Information for anti-ENPP7 antibody
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase/phosphodiesterase family. ENPP7/NPP-7 is expressed in the intestines and human bile. ENPP7/NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7/NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7/NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7/NPP-7 may be associated with human colon cancer.
Product Categories/Family for anti-ENPP7 antibody
References
Wu J. et al., 2005, Biochem. J. 386:153-60. Wu J. et al., 2004, Carcinogenesis 25:1327-33. Duan R.-D. et al., 2003, J. Lipid Res. 44:1241-50. Duan R.-D. et al., 2003, J. Biol. Chem. 278:38528-36.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,494 Da
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 7
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 7
NCBI Official Symbol
ENPP7
NCBI Official Synonym Symbols
MGC50179; ALK-SMase
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 7; NPP-7; E-NPP 7; alkaline sphingomyelinase; intestinal alkaline sphingomyelinase; alkaline sphingomyelin phosphodiesterase
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7
Protein Family
UniProt Gene Name
ENPP7
UniProt Synonym Gene Names
E-NPP 7; NPP-7; Alk-SMase
UniProt Entry Name
ENPP7_HUMAN
Uniprot Description
ENPP7: Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: Phosphodiesterase; Lipid Metabolism - sphingolipid; Cell cycle regulation; DNA replication; Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.1.4.12
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: Golgi apparatus; microvillus; membrane; integral to membrane; plasma membrane
Molecular Function: sphingomyelin phosphodiesterase activity
Biological Process: negative regulation of cell proliferation; sphingomyelin metabolic process; sphingolipid metabolic process; negative regulation of DNA replication; glycosphingolipid metabolic process; sphingomyelin catabolic process
Research Articles on ENPP7
Similar Products
Product Notes
The ENPP7 enpp7 (Catalog #AAA8104236) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ENPP7 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPP7 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:20-1:100. Researchers should empirically determine the suitability of the ENPP7 enpp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPP7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
