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Western Blot (WB) (ENPEP Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ENPEP antibody detected the ENPEP protein (arrow).)

Rabbit anti-Human ENPEP Polyclonal Antibody | anti-ENPEP antibody

ENPEP Antibody (C-term)

Gene Names
ENPEP; APA; CD249; gp160
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ENPEP; Polyclonal Antibody; ENPEP Antibody (C-term); Glutamyl aminopeptidase; EAP; Aminopeptidase A; AP-A; Differentiation antigen gp160; CD249; anti-ENPEP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ENPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-930 amino acids from the C-terminal region of human ENPEP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
899-930
Sequence Length
957
Applicable Applications for anti-ENPEP antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ENPEP Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ENPEP antibody detected the ENPEP protein (arrow).)

Western Blot (WB) (ENPEP Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ENPEP antibody detected the ENPEP protein (arrow).)

Immunohistochemistry (IHC)

(ENPEP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENPEP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ENPEP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENPEP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ENPEP Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ENPEP Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ENPEP antibody
ENPEP appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
Product Categories/Family for anti-ENPEP antibody
References
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Perez, I., et al. Head Neck 31(10):1335-1340(2009)
Sevalle, J., et al. J. Neurochem. 109(1):248-256(2009)
Teranishi, J., et al. Prostate 68(15):1666-1673(2008)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
109244
NCBI Official Full Name
glutamyl aminopeptidase
NCBI Official Synonym Full Names
glutamyl aminopeptidase (aminopeptidase A)
NCBI Official Symbol
ENPEP
NCBI Official Synonym Symbols
APA; CD249; gp160
NCBI Protein Information
glutamyl aminopeptidase
UniProt Protein Name
Glutamyl aminopeptidase
UniProt Gene Name
ENPEP
UniProt Synonym Gene Names
EAP; AP-A
UniProt Entry Name
AMPE_HUMAN

Uniprot Description

ENPEP: Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. Belongs to the peptidase M1 family.

Protein type: Protease; EC 3.4.11.7; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: integral to plasma membrane; lysosomal membrane; apical part of cell; apical plasma membrane; plasma membrane; cytoplasmic vesicle; brush border; external side of plasma membrane

Molecular Function: metallopeptidase activity; zinc ion binding; aminopeptidase activity

Biological Process: cell proliferation; cell migration; cellular protein metabolic process; cell-cell signaling; angiotensin catabolic process in blood; angiogenesis; proteolysis; angiotensin maturation; regulation of systemic arterial blood pressure by renin-angiotensin; glomerulus development

Research Articles on ENPEP

Similar Products

Product Notes

The ENPEP enpep (Catalog #AAA9203492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 899-930. The ENPEP Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPEP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ENPEP enpep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENPEP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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