Rabbit anti-Human ENG Polyclonal Antibody | anti-ENG antibody

ENG, ID (ENG, END, Endoglin, CD105) (APC)

Cat. Num. AAA6295590

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ENG; Polyclonal Antibody; ID (ENG; END; Endoglin; CD105) (APC); CD105; anti-ENG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ENG antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ENG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-302 amino acids from the Central region of human ENG.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ENG (arrow) using rabbit polyclonal ENG Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ENG gene.)

Flow Cytometry (FC/FACS)

(ENG Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ENG antibody

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-ENG antibody

Antibodies; CD Markers

References

Lopez-Novoa, J.M., et al. Am. J. Physiol. Heart Circ. Physiol. 299 (4), H959-H974 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Zabaneh, D., et al. PLoS ONE 5 (8), E11961 (2010): Maynard, S.E., et al. Hypertens Pregnancy 29(3):330-341(2010). Rius, C., et al. Blood 92(12):4677-4690(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 168693647
• NCBI GeneID: 2022
• NCBI Accession #: NP_001108225.1
• NCBI GenBank Nucleotide #: NM_001114753.2
• UniProt Accession #: P17813; Q14248; Q14926; Q5T9C0
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: endoglin isoform 1
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin; CD105 antigen
• UniProt Protein Name: Endoglin
• Protein Family: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]