Rabbit anti-Human, mouse CD105 Polyclonal Antibody | anti-ENG antibody

CD105 Antibody (Center E395)

Cat. Num. AAA9204390

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CD105; Polyclonal Antibody; CD105 Antibody (Center E395); Endoglin; ENG; END; anti-ENG antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CD105 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-409 amino acids from the Central region of human CD105.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 380-409
• Sequence Length: 625

• Applicable Applications for anti-ENG antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of CD105 antibody (Center E395) in mouse heart tissue lysates (35ug/lane). CD105 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CD105 antibody (Center E395) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD105 antibody (Center E395) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using CD105 Antibody (Center E395)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ENG antibody

CD105 is a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in its gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.

Product Categories/Family for anti-ENG antibody

Cancer; Cardiovascular; Developmental Biology; Immunology; Signal Transduction

References

Chen,Y., Ann. Neurol. 66 (1), 19-27 (2009)
Rius,C., Blood 92 (12), 4677-4690 (1998)

NCBI and Uniprot Product Information

• NCBI GI #: 4557555
• NCBI GeneID: 2022
• NCBI Accession #: NP_000109.1; NP_001108225.1; NP_001265067.1
• NCBI GenBank Nucleotide #: NM_000118.3
• UniProt Accession #: P17813; Q14248; Q14926; Q5T9C0
• Molecular Weight: 70578
• NCBI Official Full Name: endoglin isoform 2
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin
• UniProt Protein Name: Endoglin
• Protein Family: Endoglin
• UniProt Gene Name: ENG
• UniProt Synonym Gene Names: END
• UniProt Entry Name: EGLN_HUMAN

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

Uniprot Description

ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: nucleoplasm; extracellular space; cell surface; focal adhesion; cytoplasm; receptor complex; external side of plasma membrane

Molecular Function: transforming growth factor beta receptor activity; protein binding; transmembrane receptor activity; protein homodimerization activity; glycosaminoglycan binding; transforming growth factor beta binding; galactose binding; punt binding; activin binding; transforming growth factor beta receptor, cytoplasmic mediator activity

Biological Process: wound healing; positive regulation of collagen biosynthetic process; cell motility involved in cell locomotion; positive regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; response to corticosteroid stimulus; BMP signaling pathway; extracellular matrix disassembly; smooth muscle development; regulation of transforming growth factor beta receptor signaling pathway; central nervous system vasculogenesis; venous blood vessel morphogenesis; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; heart looping; cell adhesion; chronological cell aging; vasculogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of cell adhesion; cell migration; negative regulation of nitric-oxide synthase activity; regulation of phosphorylation; regulation of cell proliferation; patterning of blood vessels; negative regulation of endothelial cell proliferation; response to hypoxia; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway

Disease: Telangiectasia, Hereditary Hemorrhagic, Of Rendu, Osler, And Weber