Human Calmodulin (CAM) ELISA Kit | CAM elisa kit

Human Calmodulin (CAM) ELISA Kit

Cat. Num. AAA264769

• Gene Names: CALM1; caM; CAMI; PHKD; CPVT4; DD132; CALML2
• Reactivity: Human
• Synonyms: Calmodulin (CAM); Human Calmodulin (CAM) ELISA Kit; CAM elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 149

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant CAM concentration
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAM elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human CAM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 5901912
• NCBI GeneID: 801
• NCBI Accession #: NP_008819.1
• NCBI GenBank Nucleotide #: NM_006888.4
• Molecular Weight: 16,838 Da
• NCBI Official Full Name: calmodulin
• NCBI Official Synonym Full Names: calmodulin 1 (phosphorylase kinase, delta)
• NCBI Official Symbol: CALM1
• NCBI Official Synonym Symbols: caM; CAMI; PHKD; CPVT4; DD132; CALML2
• NCBI Protein Information: calmodulin; prepro-calmodulin 1; phosphorylase kinase, delta subunit
• UniProt Protein Name: Calmodulin
• Protein Family: Calmodulin
• UniProt Gene Name: CALM1
• UniProt Synonym Gene Names: CALM; CAM; CAM1; CALM2; CAM2; CAMB; CALM3; CALML2; CAM3; CAMC; CAMIII; CaM
• UniProt Entry Name: CALM_HUMAN

NCBI Description

This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; extracellular region; plasma membrane; cytosol; nucleus; vesicle

Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; calcium-dependent protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein binding; N-terminal myristoylation domain binding; phospholipase binding; thioesterase binding; phosphoinositide 3-kinase binding; type 3 metabotropic glutamate receptor binding; nitric-oxide synthase binding; adenylate cyclase binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; phototransduction, visible light; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; pathogenesis; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; platelet degranulation; substantia nigra development; muscle contraction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; platelet activation; glycogen catabolic process; fibroblast growth factor receptor signaling pathway; regulation of heart rate; positive regulation of nitric-oxide synthase activity; organelle organization and biogenesis; adenylate cyclase activation; response to amphetamine; glucose metabolic process; regulation of cytokinesis; positive regulation of protein amino acid autophosphorylation; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; detection of calcium ion; regulation of nitric-oxide synthase activity; response to calcium ion; blood coagulation; vascular endothelial growth factor receptor signaling pathway

Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4