Rabbit Endoplasmic reticulum resident protein 29 Polyclonal Antibody | anti-ERP29 antibody

Rabbit anti-human Endoplasmic reticulum resident protein 29 polyclonal Antibody, HRP conjugated

Cat. Num. AAA715862

• Gene Names: ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
• Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Hamster
• Applications: ELISA
• Purity: Caprylic Acid Ammonium Sulfate Precipitation
• Synonyms: Endoplasmic reticulum resident protein 29; Polyclonal Antibody; Rabbit anti-human Endoplasmic reticulum resident protein 29 polyclonal Antibody; HRP conjugated; anti-ERP29 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Hamster
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation
• Sequence Length: 53

• Applicable Applications for anti-ERP29 antibody: ELISA (EIA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: HRP
• Immunogen: Recombinant human Endoplasmic reticulum resident protein 29 protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49656 / sc-49658
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blot All lanes: Endoplasmic reticulum resident protein 29 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa Additional bands at: 33 kDa, 55kDa; We are unsure as to the identity of these extra bands.)

Related Product Information for anti-ERP29 antibody

Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI's thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.

NCBI and Uniprot Product Information

• NCBI GI #: 77628151
• NCBI GeneID: 10961
• NCBI Accession #: NP_001029197.1
• NCBI GenBank Nucleotide #: NM_001034025.1
• UniProt Accession #: P30040; Q3MJC3; Q6FHT4; C9J183
• Molecular Weight: 5,477 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 29 isoform 2
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 29
• NCBI Official Symbol: ERP29
• NCBI Official Synonym Symbols: ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
• NCBI Protein Information: endoplasmic reticulum resident protein 29
• UniProt Protein Name: Endoplasmic reticulum resident protein 29
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP29
• UniProt Synonym Gene Names: C12orf8; ERP28; ERp29; ERp28; ERp31
• UniProt Entry Name: ERP29_HUMAN

NCBI Description

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

ERP29: Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; melanosome; membrane; smooth endoplasmic reticulum; transport vesicle

Molecular Function: chaperone binding; protein binding; protein disulfide isomerase activity; protein homodimerization activity

Biological Process: activation of MAPK activity; intracellular protein transport; negative regulation of protein secretion; positive regulation of protein amino acid phosphorylation; protein folding; protein secretion; protein unfolding