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Western Blot (WB) (HeLa cells were subjected to SDS PAGE followed by western blot with EMB Polyclonal Antibody at dilution of 1:1000)

Rabbit anti-Human, Mouse EMB Polyclonal Antibody | anti-EMB antibody

EMB Antibody

Gene Names
EMB; GP70
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
EMB; Polyclonal Antibody; EMB Antibody; Embigin; embigin homolog (mouse); anti-EMB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Form/Format
PBS with 0.1% sodium azide, 50% glycerol, pH 7.3
Sequence Length
327
Applicable Applications for anti-EMB antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:20-1:200
Immunogen
Fusion protein of EMB
Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with EMB Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (HeLa cells were subjected to SDS PAGE followed by western blot with EMB Polyclonal Antibody at dilution of 1:1000)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffinembedded human testis tissue slide using EMB Polyclonal Antibody at dilution of 1:50 (under 40x lens))

Immunohistochemistry (IHC) (Immunohistochemistry of paraffinembedded human testis tissue slide using EMB Polyclonal Antibody at dilution of 1:50 (under 40x lens))
Related Product Information for anti-EMB antibody
EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated Molecular Weight: 37kDa
Observed Molecular Weight: 70kDa
NCBI Official Full Name
Embigin
NCBI Official Synonym Full Names
embigin
NCBI Official Symbol
EMB
NCBI Official Synonym Symbols
GP70
NCBI Protein Information
embigin; embigin homolog
UniProt Protein Name
Embigin
Protein Family
UniProt Gene Name
EMB
UniProt Entry Name
EMB_HUMAN

NCBI Description

This gene encodes a transmembrane glycoprotein that is a member of the immunoglobulin superfamily. The encoded protein may be involved in cell growth and development by mediating interactions between the cell and extracellular matrix. A pseudogene of this gene is found on chromosome 1. [provided by RefSeq, Jan 2009]

Uniprot Description

EMB: May function as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: integral to plasma membrane; synapse; cell junction

Biological Process: cell adhesion

Research Articles on EMB

Similar Products

Product Notes

The EMB emb (Catalog #AAA2525723) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EMB Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EMB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the EMB emb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EMB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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