Western Blot (WB)
(Western blot analysis of ELOF1 using HuvEc whole cell lysates)
Rabbit ELOF1 Polyclonal Antibody | anti-ELOF1 antibody
ELOF1 Antibody
Gene Names
ELOF1; ELF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
ELOF1; Polyclonal Antibody; ELOF1 Antibody; ELF1; elof1; ELOF1_HUMAN; Elongation factor 1 homolog (ELF1; S. cerevisiae); Elongation factor 1 homolog (S. cerevisiae); Transcription elongation factor 1 homolog; anti-ELOF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
ELOF1 Antibody detects endogenous levels of total ELOF1.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ELOF1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB 1:1000-3000
IHC 1:200
ELISA(peptide)1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC 1:200
ELISA(peptide)1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human ELOF1,corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of ELOF1 using HuvEc whole cell lysates)
Western Blot (WB)
(Western blot analysis of ELOF1 using HuvEc whole cell lysates)
Immunohistochemistry (IHC)
(MBS9610282 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9610282 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ELOF1 antibody
Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.
Similarity: Belongs to the ELOF1 family.
Similarity: Belongs to the ELOF1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9 kDa
NCBI Official Full Name
transcription elongation factor 1 homolog isoform 1
NCBI Official Synonym Full Names
elongation factor 1 homolog
NCBI Official Symbol
ELOF1
NCBI Official Synonym Symbols
ELF1
NCBI Protein Information
transcription elongation factor 1 homolog
UniProt Protein Name
Transcription elongation factor 1 homolog
Protein Family
UniProt Gene Name
ELOF1
Uniprot Description
Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.
Similar Products
Product Notes
The ELOF1 elof1 (Catalog #AAA9610282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ELOF1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ELOF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB 1:1000-3000 IHC 1:200 ELISA(peptide)1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the ELOF1 elof1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELOF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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