Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using TRA-2alpha antibody.)
Rabbit anti-Human, Mouse TRA-2alpha Polyclonal Antibody | anti-TRA2A antibody
TRA-2alpha Antibody
Gene Names
TRA2A; AWMS1; HSU53209
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
TRA-2alpha; Polyclonal Antibody; TRA-2alpha Antibody; AWMS1; HSU53209; htra-2 alpha; Putative MAPK activating protein PM24; TRA 2 alpha; TRA 2A; TRA-2 alpha; TRA2 alpha; TRA2-alpha; TRA2A; TRA2A_HUMAN; Tra2alpha; Transformer 2 alpha; Transformer 2 protein homolog; Transformer-2 protein homolog A; Transformer-2 protein homolog alpha; anti-TRA2A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TRA-2alpha antibody detects endogenous levels of total TRA-2alpha
Purity/Purification
Peptide affinity purification
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
181
Applicable Applications for anti-TRA2A antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
ELISA: 1:20000-1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human TRA-2 alpha, corresponding to a region within C-terminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20°C. Stable for up to 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using TRA-2alpha antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HUVEC cells, using TRA-2alpha antibody.)
Immunohistochemistry (IHC)
(MBS9604731 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604731 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF)
(MBS9604731 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9604731 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-TRA2A antibody
Function: Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Subunit Structure: Binds to A3 enhancer proteins SRp75, SRp55, SRp40 and SRp30.
Post-translational Modifications: Phosphorylated in the RS domains.
Similarity: Belongs to the splicing factor SR family.
Subunit Structure: Binds to A3 enhancer proteins SRp75, SRp55, SRp40 and SRp30.
Post-translational Modifications: Phosphorylated in the RS domains.
Similarity: Belongs to the splicing factor SR family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33 kD
NCBI Official Full Name
transformer-2 protein homolog alpha isoform 2
NCBI Official Synonym Full Names
transformer 2 alpha homolog
NCBI Official Symbol
TRA2A
NCBI Official Synonym Symbols
AWMS1; HSU53209
NCBI Protein Information
transformer-2 protein homolog alpha
UniProt Protein Name
Transformer-2 protein homolog alpha
Protein Family
UniProt Gene Name
TRA2A
UniProt Synonym Gene Names
TRA-2 alpha; TRA2-alpha
NCBI Description
This gene is a member of the transformer 2 homolog family and encodes a protein with several RRM (RNA recognition motif) domains. This phosphorylated nuclear protein binds to specific RNA sequences and plays a role in the regulation of pre-mRNA splicing. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]
Uniprot Description
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Research Articles on TRA2A
Similar Products
Product Notes
The TRA2A tra2a (Catalog #AAA9604731) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRA-2alpha Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRA-2alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500 ELISA: 1:20000-1:40000 The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the TRA2A tra2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRA-2alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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