anti-Human, Mouse RAB12 Antibody | anti-RAB12 antibody

RAB12 Antibody

Cat. Num. AAA9603687

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Peptide affinity purification
• Synonyms: RAB12; RAB12 Antibody; Putative Ras related protein Rab 12; member RAS oncogene family; RAB12_HUMAN; Ras-related protein Rab-12; anti-RAB12 antibody

• Reactivity: Human, Mouse
• Specificity: RAB12 Antibody detects endogenous levels of total RAB12
• Purity/Purification: Peptide affinity purification
• Form/Format: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RAB12 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (Peptide)
• Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide)1:20000-1:40000; *The optimal dilutions should be determined by the end user.; ; IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking,overnight.
• Immunogen: A synthesized peptide derived from human RAB12,corresponding to a region within C-terminal amino acids.
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603687 at 1/100 staining Human pancreas cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts from B16F10, using RAB12Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-RAB12 antibody

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 106507164
• NCBI GeneID: 201475
• NCBI Accession #: NP_001020471.2
• NCBI GenBank Nucleotide #: NM_001025300.2
• UniProt Accession #: Q6IQ22; Q4KMQ3; A6NEF5
• Molecular Weight: 28 kDa
• NCBI Official Full Name: ras-related protein Rab-12
• NCBI Official Synonym Full Names: RAB12, member RAS oncogene family
• NCBI Official Symbol: RAB12
• NCBI Protein Information: ras-related protein Rab-12
• UniProt Protein Name: Ras-related protein Rab-12
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB12

Uniprot Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy ().