Rabbit ELL Polyclonal Antibody | anti-ELL antibody

ELL (D125) Polyclonal Antibody

Cat. Num. AAA3002057

• Gene Names: ELL; MEN; ELL1; PPP1R68; C19orf17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ELL; Polyclonal Antibody; ELL (D125) Polyclonal Antibody; RNA polymerase II elongation factor ELL; Eleven-nineteen lysine-rich leukemia protein; C19orf17; anti-ELL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ELL (D125) polyclonal antibody detects endogenous levels of ELL protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 621

• Applicable Applications for anti-ELL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human ELL.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ELL (D125) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ELL (D125) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ELL (D125) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ELL antibody

ELL was shown to encode an elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. ELL is the second elongation factor to be implicated in oncogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 5729812
• NCBI GeneID: 8178
• NCBI Accession #: NP_006523.1
• NCBI GenBank Nucleotide #: NP_006523.1
• UniProt Accession #: P55199
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: RNA polymerase II elongation factor ELL
• NCBI Official Synonym Full Names: elongation factor for RNA polymerase II
• NCBI Official Symbol: ELL
• NCBI Official Synonym Symbols: MEN; ELL1; PPP1R68; C19orf17
• NCBI Protein Information: RNA polymerase II elongation factor ELL
• UniProt Protein Name: RNA polymerase II elongation factor ELL
• Protein Family: ELL-associated factor
• UniProt Gene Name: ELL
• UniProt Synonym Gene Names: C19orf17
• UniProt Entry Name: ELL_HUMAN

Uniprot Description

ELL: Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. A chromosomal aberration involving ELL is found in acute leukemias. Translocation t(11;19)(q23;p13.1) with MLL/HRX. The result is a rogue activator protein. Belongs to the ELL/occludin family.

Protein type: Transcription initiation complex; Nuclear receptor co-regulator; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; Cajal body; transcription elongation factor complex; nuclear speck

Molecular Function: protein binding; phosphatase binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of viral transcription; in utero embryonic development; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; gene expression; snRNA transcription from RNA polymerase III promoter