Rabbit anti-Human, Mouse ELL Polyclonal Antibody | anti-ELL antibody

ELL, CT (ELL, C19orf17, RNA polymerase II elongation factor ELL, Eleven-nineteen lysine-rich leukemia protein) (Biotin)

Cat. Num. AAA6295261

• Gene Names: ELL; MEN; ELL1; PPP1R68; C19orf17
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ELL; Polyclonal Antibody; CT (ELL; C19orf17; RNA polymerase II elongation factor ELL; Eleven-nineteen lysine-rich leukemia protein) (Biotin); Eleven-nineteen lysine-rich leukemia protein; anti-ELL antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ELL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ELL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 523~553 amino acids from the C-terminal region of human ELL.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELL Antibody (C-term) in mouse testis tissue lysates (35ug/lane). ELL (arrow) was detected using the purified Pab.)

Related Product Information for anti-ELL antibody

Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.

Product Categories/Family for anti-ELL antibody

Antibodies; Cancer Markers; Leukemia

References

Zhou, J., et al. J. Biol. Chem. 284(28):19142-19152(2009). Jiang, F., et al. Cancer Lett. 253(2):265-272(2007). Matsuoka., S., et al. Science 316(5828):1160-1166(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5729812
• NCBI GeneID: 8178
• NCBI Accession #: NP_006523.1
• NCBI GenBank Nucleotide #: NM_006532.3
• UniProt Accession #: P55199
• Molecular Weight: 68,265 Da
• NCBI Official Full Name: RNA polymerase II elongation factor ELL
• NCBI Official Synonym Full Names: elongation factor RNA polymerase II
• NCBI Official Symbol: ELL
• NCBI Official Synonym Symbols: MEN; ELL1; PPP1R68; C19orf17
• NCBI Protein Information: RNA polymerase II elongation factor ELL; ELL gene (11-19 lysine-rich leukemia gene); eleven-nineteen lysine-rich leukemia protein; protein phosphatase 1, regulatory subunit 68
• UniProt Protein Name: RNA polymerase II elongation factor ELL
• Protein Family: ELL-associated factor
• UniProt Gene Name: ELL
• UniProt Synonym Gene Names: C19orf17
• UniProt Entry Name: ELL_HUMAN

Uniprot Description

ELL: Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. A chromosomal aberration involving ELL is found in acute leukemias. Translocation t(11;19)(q23;p13.1) with MLL/HRX. The result is a rogue activator protein. Belongs to the ELL/occludin family.

Protein type: Transcription initiation complex; Nuclear receptor co-regulator; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleoplasm; Cajal body; transcription elongation factor complex; nuclear speck

Molecular Function: protein binding; phosphatase binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of viral transcription; in utero embryonic development; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; gene expression; snRNA transcription from RNA polymerase III promoter