Rabbit anti-Human COL8A1 Polyclonal Antibody | anti-COL8A1 antibody

COL8A1, NT (COL8A1, C3orf7, Collagen alpha-1(VIII) chain, Endothelial collagen, Vastatin) (MaxLight 490)

Cat. Num. AAA6287752

• Gene Names: COL8A1; C3orf7
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: COL8A1; Polyclonal Antibody; NT (COL8A1; C3orf7; Collagen alpha-1(VIII) chain; Endothelial collagen; Vastatin) (MaxLight 490); Vastatin; anti-COL8A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COL8A1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-COL8A1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 62-89 in the N-terminal region of human COL8A1.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB453 cell line lysates (35ug/lane) usingMBS643158. This demonstrates that MBS643158 detected the COL8A1 protein (arrow).)

Western Blot (WB)

(Western Blot using MBS643158 [1:1000]. Lane 1: Human heart lysate Lane 2: human liver lysate (20ug/lane) Secondary IgG (HRP) goat anti-rabbit (1:10000). Predicted band size: 73kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-COL8A1 antibody

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo-or a heterotrimer. Alternatively spliced transcript variants encoding the same isoform have been observed.

Product Categories/Family for anti-COL8A1 antibody

Antibodies; KIAA; orf

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Neale, B.M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(16):7395-7400(2010). 4. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 5. Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 17738302
• NCBI GeneID: 1295
• NCBI Accession #: NP_001841.2; NP_065084.2
• NCBI GenBank Nucleotide #: NM_001850.4
• UniProt Accession #: P27658; Q53XI6; Q96D07; D3DN42
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: collagen alpha-1(VIII) chain
• NCBI Official Synonym Full Names: collagen type VIII alpha 1
• NCBI Official Symbol: COL8A1
• NCBI Official Synonym Symbols: C3orf7
• NCBI Protein Information: collagen alpha-1(VIII) chain
• UniProt Protein Name: Collagen alpha-1(VIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL8A1
• UniProt Synonym Gene Names: C3orf7
• UniProt Entry Name: CO8A1_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]

Uniprot Description

COL8A1: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q12.3

Cellular Component: extracellular matrix; intracellular membrane-bound organelle; endoplasmic reticulum lumen; extracellular region; collagen type VIII

Biological Process: collagen catabolic process; extracellular matrix disassembly; epithelial cell proliferation; extracellular matrix organization and biogenesis; camera-type eye morphogenesis; angiogenesis; cell adhesion