Western Blot (WB)
(Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane). EIF4E2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human EIF4E2 Polyclonal Antibody | anti-EIF4E2 antibody
EIF4E2 (Eukaryotic Translation Initiation Factor 4E Type 2, eIF4E Type 2, eIF-4E Type 2, mRNA Cap-binding Protein Type 3, Eukaryotic Translation Initiation Factor 4E-like 3, Eukaryotic Translation Initiation Factor 4E Homologous Protein, mRNA Cap-binding
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Western Blot (WB)
(Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane). EIF4E2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane). EIF4E2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of EIF4E2 (arrow) using rabbit polyclonal EIF4E2 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.)
Western Blot (WB)
(Western blot analysis of EIF4E2 (arrow) using rabbit polyclonal EIF4E2 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
Uniprot Description
EIF4E2: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners. EIF4E2 interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 but not with eIF4G. Belongs to the eukaryotic initiation factor 4E family.
Protein type: Translation initiation; Translation
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: mRNA cap complex; cytosol
Molecular Function: protein binding; translation factor activity, nucleic acid binding; ubiquitin protein ligase binding; translation initiation factor activity; RNA cap binding
Biological Process: in utero embryonic development; negative regulation of translation; cytokine and chemokine mediated signaling pathway; translational initiation
Research Articles on EIF4E2
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Product Notes
The EIF4E2 eif4e2 (Catalog #AAA630035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF4E2 (Eukaryotic Translation Initiation Factor 4E Type 2, eIF4E Type 2, eIF-4E Type 2, mRNA Cap-binding Protein Type 3, Eukaryotic Translation Initiation Factor 4E-like 3, Eukaryotic Translation Initiation Factor 4E Homologous Protein, mRNA Cap-binding reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4E2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the EIF4E2 eif4e2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4E2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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