Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Speckle-type POZ protein-like (SPOPL) Standard Curve.)
Human Speckle-Type POZ Protein-Like (SPOPL) ELISA Kit | SPOPL elisa kit
Human Speckle-Type POZ Protein-Like (SPOPL) ELISA Kit
Gene Names
SPOPL; BTBD33
Reactivity
Human
Synonyms
Speckle-Type POZ Protein-Like (SPOPL); Human Speckle-Type POZ Protein-Like (SPOPL) ELISA Kit; SPOPL; FLJ53775; HIB homolog 2; roadkill homolog 2; SPOPL elisa kit
Reactivity
Human
Specificity
Human Speckle-type POZ protein-like (SPOPL) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPOPL. No significant cross-reactivity or interference between Human SPOPL and analogues was observed.
Sequence Length
392
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Speckle-type POZ protein-like (SPOPL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Speckle-type POZ protein-like (SPOPL) Standard Curve.)
Related Product Information for SPOPL elisa kit
Description: This Human Speckle-type POZ protein-like (SPOPL) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOPL in samples. An antibody specific for SPOPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPOPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPOPL Belongs to the Tdpoz family.Contains 1 BTB (POZ) domain.Contains 1 MATH domain.TDPOZ, a family of bipartite animal and plant proteins that contain the TRAF (TD) and POZ/BTB domains. The Tdpoz gene cluster is mapped on chromosome 3 at 3F2.1-2.2. RT-PCR experiments and a search of expressed sequence tag (EST) databases show that the Tdpoz1-5 genes are transcribed in early embryos, particularly at the two-cell stage. TDPOZ proteins form a new protein family on the basis of similar protein domain organization. TDPOZ proteins may be nuclear scaffold proteins probably involved in transcription regulation in early development and other cellular processes.
Background: SPOPL Belongs to the Tdpoz family.Contains 1 BTB (POZ) domain.Contains 1 MATH domain.TDPOZ, a family of bipartite animal and plant proteins that contain the TRAF (TD) and POZ/BTB domains. The Tdpoz gene cluster is mapped on chromosome 3 at 3F2.1-2.2. RT-PCR experiments and a search of expressed sequence tag (EST) databases show that the Tdpoz1-5 genes are transcribed in early embryos, particularly at the two-cell stage. TDPOZ proteins form a new protein family on the basis of similar protein domain organization. TDPOZ proteins may be nuclear scaffold proteins probably involved in transcription regulation in early development and other cellular processes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
speckle-type POZ protein-like
NCBI Official Synonym Full Names
speckle type BTB/POZ protein like
NCBI Official Symbol
SPOPL
NCBI Official Synonym Symbols
BTBD33
NCBI Protein Information
speckle-type POZ protein-like
UniProt Protein Name
Speckle-type POZ protein-like
Protein Family
UniProt Gene Name
SPOPL
UniProt Entry Name
SPOPL_HUMAN
Uniprot Description
SPOPL: In complex with a cullin, may act in ubiquitination and proteasomal degradation processes. Belongs to the Tdpoz family.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 2q22.1
Cellular Component: nucleus
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; negative regulation of protein ubiquitination
Research Articles on SPOPL
Similar Products
Product Notes
The Human SPOPL spopl (Catalog #AAA9714007) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714007 ELISA Kit recognizes Human SPOPL. It is sometimes possible for the material contained within the vial of "Speckle-Type POZ Protein-Like (SPOPL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
