Rabbit eIF4E Polyclonal Antibody | anti-eIF4E antibody

eIF4E (Phospho-S209) Polyclonal Antibody

Cat. Num. AAA3005260

• Gene Names: EIF4E; CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: eIF4E; Polyclonal Antibody; eIF4E (Phospho-S209) Polyclonal Antibody; eIF4E (phospho S209); eIF-4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; 1977; 133440; P06730; CBP; EIF4EL1; EIF4F; MGC111573; anti-eIF4E antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-eIF4E (S209) polyclonal antibody detects endogenous levels of eIF4E protein only when phosphorylated at Ser209.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 237

• Applicable Applications for anti-eIF4E antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Serine 209.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-eIF4E (S209) pAb at 1:500 dilutionLane1:The Lung tissue lysate of Mouse (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:3T3-L1 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:A2780 whole cell lysate (40ug) Lane6:SK-OVCAR3 whole cell lysate (40ug) Lane7:Panc1 whole cell lysate (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-eIF4E (S209) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-eIF4E antibody

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

NCBI and Uniprot Product Information

• NCBI GI #: 194578907
• NCBI GeneID: 1977
• NCBI Accession #: NP_001124150.1
• NCBI GenBank Nucleotide #: NP_001124150.1
• UniProt Accession #: P06730
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 4E isoform 3
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E
• NCBI Official Symbol: EIF4E
• NCBI Official Synonym Symbols: CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
• NCBI Protein Information: eukaryotic translation initiation factor 4E
• UniProt Protein Name: Eukaryotic translation initiation factor 4E
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4E
• UniProt Synonym Gene Names: EIF4EL1; EIF4F; eIF-4E; eIF4E
• UniProt Entry Name: IF4E_HUMAN

NCBI Description

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: mRNA cap complex; stress granule; cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding

Biological Process: translation; viral reproduction; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; lung development; G1/S transition of mitotic cell cycle

Disease: Autism, Susceptibility To, 19