Mouse anti-Human EIF4E Antibody | anti-EIF4E antibody

EIF4E Antibody

Cat. Num. AAA850484

• Gene Names: EIF4E; CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Synonyms: EIF4E; EIF4E Antibody; CBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; anti-EIF4E antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: 5D11
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 237

• Applicable Applications for anti-EIF4E antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000
• Immunogen: Purified recombinant fragment of human EIF4E expressed in E. Coli.
• Preparation and Storage: Store at 4 degree C, for long term storage, store at-20 degree C.

Western Blot (WB)

(Figure 1: Western blot analysis using EIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.)

Immunohistochemistry (IHC)

(Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using EIF4E mouse mAb with DAB staining.)

Testing Data

Related Product Information for anti-EIF4E antibody

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Product Categories/Family for anti-EIF4E antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

NCBI and Uniprot Product Information

• NCBI GI #: 194578907
• NCBI GeneID: 1977
• NCBI Accession #: NP_001124150.1
• NCBI GenBank Nucleotide #: NM_001130678.2
• UniProt Accession #: P06730; Q96E95; B7Z6V1; D6RCQ6
• Molecular Weight: 25kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 4E isoform 3
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E
• NCBI Official Symbol: EIF4E
• NCBI Official Synonym Symbols: CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
• NCBI Protein Information: eukaryotic translation initiation factor 4E
• UniProt Protein Name: Eukaryotic translation initiation factor 4E
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4E
• UniProt Synonym Gene Names: EIF4EL1; EIF4F; eIF-4E; eIF4E

NCBI Description

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.