Goat anti-Human FOXO3 Polyclonal Antibody | anti-FOXO3 antibody

FOXO3, CT (Forkhead Box Protein O3, AF6q21 Protein, Forkhead in Rhabdomyosarcoma-like 1, FKHRL1, FOXO3A)

Cat. Num. AAA644676

• Gene Names: FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: FOXO3; Polyclonal Antibody; CT (Forkhead Box Protein O3; AF6q21 Protein; Forkhead in Rhabdomyosarcoma-like 1; FKHRL1; FOXO3A); Anti -FOXO3; anti-FOXO3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human FOXO3. Species sequence homology: Bovine, canine, mouse, porcine, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-FOXO3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 0.1-0.3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to GAKQASSQSWVPG from C-Terminus of human FKHRL1/FOXO3A conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Staining (0.5ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.)

Product Categories/Family for anti-FOXO3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 42519916
• NCBI GeneID: 2309
• NCBI Accession #: NP_963853.1
• NCBI GenBank Nucleotide #: NM_201559.2
• UniProt Accession #: O43524; O15171; Q5T2I7; Q9BZ04; E1P5E6
• Molecular Weight: 71,277 Da
• NCBI Official Full Name: forkhead box protein O3
• NCBI Official Synonym Full Names: forkhead box O3
• NCBI Official Symbol: FOXO3
• NCBI Official Synonym Symbols: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• NCBI Protein Information: forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• UniProt Protein Name: Forkhead box protein O3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO3
• UniProt Synonym Gene Names: FKHRL1; FOXO3A
• UniProt Entry Name: FOXO3_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: Transcription factor; DNA-binding; Oncoprotein; Autophagy

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; initiation of primordial ovarian follicle growth; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; antral ovarian follicle growth; negative regulation of transcription from RNA polymerase II promoter; ovulation from ovarian follicle; glucose homeostasis; oocyte maturation; regulation of transcription from RNA polymerase II promoter; regulation of translation; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter