Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated or untreated with EGF using Rabbit anti EIF4B (pSer422) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes. 3% BSA was used for blocking)
Rabbit anti-Human EIF4B Polyclonal Antibody | anti-EIF4B antibody
Rabbit Anti EIF4B (pSer422)
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated or untreated with EGF using Rabbit anti EIF4B (pSer422) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes. 3% BSA was used for blocking)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated or untreated with EGF using Rabbit anti EIF4B (pSer422) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with EGF (100ng/ml) for 30 minutes. 3% BSA was used for blocking)
NCBI and Uniprot Product Information
Uniprot Description
EIF4B: eukaryotic translation initiation factor 4B. Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Protein type: RNA-binding; Translation initiation; Translation
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: eukaryotic translation initiation factor 4F complex; cytosol
Molecular Function: protein binding; RNA binding; translation initiation factor activity; nucleotide binding
Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; regulation of translational initiation
Research Articles on EIF4B
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Product Notes
The EIF4B eif4b (Catalog #AAA225607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti EIF4B (pSer422) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the EIF4B eif4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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