Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EIF4B antibody.)
Rabbit EIF4B Polyclonal Antibody | anti-EIF4B antibody
EIF4B Antibody
Gene Names
EIF4B; EIF-4B; PRO1843
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
EIF4B; Polyclonal Antibody; EIF4B Antibody; EIF-4B; PRO1843; anti-EIF4B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total EIF4B protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
611
Applicable Applications for anti-EIF4B antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human EIF4B.
Target Name
EIF4B
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EIF4B antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EIF4B antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast cancer using EIF4B antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast cancer using EIF4B antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using EIF4B antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using EIF4B antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-EIF4B antibody
Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translational activity of eIF4B (7).
Product Categories/Family for anti-EIF4B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,805 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4B isoform 2
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4B
NCBI Official Symbol
EIF4B
NCBI Official Synonym Symbols
EIF-4B; PRO1843
NCBI Protein Information
eukaryotic translation initiation factor 4B
UniProt Protein Name
Eukaryotic translation initiation factor 4B
Protein Family
UniProt Gene Name
EIF4B
UniProt Synonym Gene Names
eIF-4B
UniProt Entry Name
IF4B_HUMAN
Uniprot Description
EIF4B: eukaryotic translation initiation factor 4B. Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Protein type: Translation initiation; Translation; RNA-binding
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: eukaryotic translation initiation factor 4F complex; cytosol
Molecular Function: protein binding; RNA binding; translation initiation factor activity; nucleotide binding
Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; insulin receptor signaling pathway; translational initiation; gene expression; regulation of translational initiation; mRNA catabolic process, deadenylation-dependent decay
Research Articles on EIF4B
Similar Products
Product Notes
The EIF4B eif4b (Catalog #AAA9408587) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF4B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the EIF4B eif4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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