Rabbit eIF3zeta Polyclonal Antibody | anti-eIF3zeta antibody

eIF3zeta (R135) Polyclonal Antibody

Cat. Num. AAA3004385

• Gene Names: EIF3D; EIF3S7; eIF3-p66; eIF3-zeta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: eIF3zeta; Polyclonal Antibody; eIF3zeta (R135) Polyclonal Antibody; Eukaryotic translation initiation factor 3 subunit D; eIF3d; Eukaryotic translation initiation factor 3 subunit 7; eIF-3-zeta; eIF3 p66; EIF3S7; EIF3D; anti-eIF3zeta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: eIF3zeta (R135) polyclonal antibody detects endogenous levels of eIF3zeta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 548

• Applicable Applications for anti-eIF3zeta antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 111-160 of Human eIF3zeta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of eIF3zeta (R135) pAb at 1:1000 dilutionLane1:The Brain tissue lysate of Mouse (40ug) Lane2:The Brain tissue lysate of Rat (20ug) Lane3:HEK293T whole cell lysate (10ug) Lane4:MCF-7 whole cell lysate (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of eIF3zeta (R135) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-eIF3zeta antibody

Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Eukaryotic initiation factor 3 (eIF3) is the largest family of eIFs and consists of at least 12 unique subunits in mammals. eIFepsilon, also known as eIF p47, binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA and associates with the complex p170-eIF3.

NCBI and Uniprot Product Information

• NCBI GI #: 4503523
• NCBI GeneID: 8664
• NCBI Accession #: NP_003744.1
• NCBI GenBank Nucleotide #: NP_003744.1
• UniProt Accession #: O15371
• Molecular Weight: ~ 64kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit D
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3 subunit D
• NCBI Official Symbol: EIF3D
• NCBI Official Synonym Symbols: EIF3S7; eIF3-p66; eIF3-zeta
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit D
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit D
• UniProt Gene Name: EIF3D
• UniProt Synonym Gene Names: EIF3S7; eIF3d
• UniProt Entry Name: EIF3D_HUMAN

NCBI Description

Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]

Uniprot Description

eIF3-zeta: eukaryotic translation initiation factor 3 subunit 7. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. Associates with the subunit p170 of eIF-3. eIF-3 is composed of at least 12 different subunits.

Protein type: Translation; RNA-binding; Translation initiation

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: eukaryotic translation initiation factor 3 complex; membrane; cytosol

Molecular Function: protein binding; translation initiation factor activity

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation initiation complex; formation of translation preinitiation complex; regulation of translational initiation