Keratin 25 Recombinant Protein | KRT25 recombinant protein

Recombinant Keratin 25 (KRT25)

Cat. Num. AAA2031827

• Gene Names: KRT25; KRT25A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Keratin 25; Recombinant Keratin 25 (KRT25); KRT25 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MSLRLSSGSR RSYARPSTGS LRGASFGAGN ACGVAGIGSG FSCAFGGSSTGGNTGVANSC AGFTVNEGGL LSGNEKVTMQ NLNDRLASYL DNVQALQEANADLEQKIKGW YEKFGPGSCR GLDHDYSRYF PIIDDLKNQI ITSTTSNANAVLQIDNARLT ADDFRLKYEN ELALHQSVEA DVNGLRRVLD EITLCRTDLEIQYETLSEEL TYLKKNHKEE MQALQCAAGG NVNVEMNAAP GVDLTVLLNNMRAEYEALAE QNRRDAEAWF QEKSASLQQQ ITEDVGATTS ARNELTEMKRTLQTLEIELQ SLLATKHSLE CSLTETEGNY CTQLAQIQAQ ISALEEQLHQVRTETEGQKL EYEQLLNVKA HLEKEIETYC LLIGGDEGAC KSSSYKSKDYGSGNAGNQIK DPVKAIVVKK VLEEVDQRSK ILTTRLHSLE EKSQSN
• Sequence Length: 450

• Applicable Applications for KRT25 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 52.6KDa
• Accurate Molecular Mass (KD): 53kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met1~Asn446
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31559829
• NCBI GeneID: 147183
• NCBI Accession #: NP_853512.1
• NCBI GenBank Nucleotide #: NM_181534.3
• Molecular Weight: 49,318 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 25
• NCBI Official Synonym Full Names: keratin 25, type I
• NCBI Official Symbol: KRT25
• NCBI Official Synonym Symbols: KRT25A
• NCBI Protein Information: keratin, type I cytoskeletal 25
• UniProt Protein Name: Keratin, type I cytoskeletal 25
• Protein Family: Keratin
• UniProt Gene Name: KRT25
• UniProt Synonym Gene Names: CK-25; K25; K25A
• UniProt Entry Name: K1C25_HUMAN

NCBI Description

This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009]

Uniprot Description

KRT25: Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs). Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: structural molecule activity

Biological Process: hair follicle morphogenesis; intermediate filament organization; aging; hair cycle