Western Blot (WB)
(Western blot analysis of EIF3H (arrow) using rabbit polyclonal EIF3H Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF3H gene.)
Rabbit anti-Human EIF3H Polyclonal Antibody | anti-LOC100190645 antibody
EIF3H, NT (EIF3H, EIF3S3, Eukaryotic translation initiation factor 3 subunit H, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit)
Gene Names
LOC100190645; EIF3H; EIF3S3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
EIF3H; Polyclonal Antibody; NT (EIF3H; EIF3S3; Eukaryotic translation initiation factor 3 subunit H; Eukaryotic translation initiation factor 3 subunit 3; eIF-3-gamma; eIF3 p40 subunit); Anti -EIF3H; anti-LOC100190645 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-LOC100190645 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
EIF3H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-99 amino acids from the N-terminal region of human EIF3H.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of EIF3H (arrow) using rabbit polyclonal EIF3H Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF3H gene.)
Western Blot (WB)
(Western blot analysis of EIF3H (arrow) using rabbit polyclonal EIF3H Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF3H gene.)
Immunohistochemistry (IHC)
(EIF3H Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3H Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(EIF3H Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3H Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-LOC100190645 antibody
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Product Categories/Family for anti-LOC100190645 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
39,529 Da
NCBI Official Full Name
Eukaryotic translation initiation factor 3 subunit H
NCBI Official Symbol
LOC100190645
NCBI Official Synonym Symbols
EIF3H; EIF3S3
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit H; eIF-3 gamma; eIF-3-gamma; eIF3 p40 subunit; putative eukaryotic translation initiation factor 3 subunit 3 gamma
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit H
UniProt Gene Name
EIF3H
UniProt Synonym Gene Names
EIF3S3; eIF3h
UniProt Entry Name
EIF3H_TAEGU
Uniprot Description
Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome
By similarity. HAMAP-Rule MF_03007
Subunit structure: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M
By similarity.
Subcellular location: Cytoplasm
By similarity HAMAP-Rule MF_03007.
Sequence similarities: Belongs to the eIF-3 subunit H family.Contains 1 MPN (JAB/Mov34) domain.
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Product Notes
The LOC100190645 eif3h (Catalog #AAA644974) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF3H, NT (EIF3H, EIF3S3, Eukaryotic translation initiation factor 3 subunit H, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3H can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the LOC100190645 eif3h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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