Rabbit anti-Human EIF3A Polyclonal Antibody | anti-eif3s10 antibody

EIF3A, CT (EIF3A, EIF3S10, KIAA0139, Eukaryotic translation initiation factor 3 subunit A, Eukaryotic translation initiation factor 3 subunit 10, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185)

Cat. Num. AAA6004833

• Gene Names: eif3s10; zgc:66194; zgc:77263; wu:fi15c03
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: EIF3A; Polyclonal Antibody; CT (EIF3A; EIF3S10; KIAA0139; Eukaryotic translation initiation factor 3 subunit A; Eukaryotic translation initiation factor 3 subunit 10; eIF-3-theta; eIF3 p167; eIF3 p180; eIF3 p185); Anti -EIF3A; anti-eif3s10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-eif3s10 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: EIF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1218-1247 amino acids from the C-terminal region of human EIF3A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EIF3A Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the EIF3A antibody detected the EIF3A protein (arrow).)

Immunohistochemistry (IHC)

(EIF3A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-eif3s10 antibody

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Product Categories/Family for anti-eif3s10 antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 45433533
• NCBI GeneID: 327515
• NCBI Accession #: NP_956114.2
• NCBI GenBank Nucleotide #: NM_199820.2
• UniProt Accession #: Q6PCR7; Q6NYB1
• Molecular Weight: 151,292 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit A
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3, subunit 10 (theta)
• NCBI Official Symbol: eif3s10
• NCBI Official Synonym Symbols: zgc:66194; zgc:77263; wu:fi15c03
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit A; eIF3a; eIF-3-theta; eukaryotic translation initiation factor 3 subunit 10
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit A
• UniProt Gene Name: eif3a
• UniProt Synonym Gene Names: eif3s10; eIF3a
• UniProt Entry Name: EIF3A_DANRE

Uniprot Description

Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

By similarity. HAMAP-Rule MF_03000

Subunit structure: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: eif3a, eif3b, eif3c, eif3d, eif3e, eif3f, eif3g, eif3h, eif3i, eif3j, eif3k, eif3l and eif3m

By similarity.

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_03000.

Sequence similarities: Belongs to the eIF-3 subunit A family.Contains 1 PCI domain.