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Western Blot (WB) (Western blot analysis of anti-ARK5 Pab in mouse brain tissue lysate. ARK5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Rabbit anti-Human, mouse ARK5 Polyclonal Antibody | anti-NUAK1 antibody

ARK5 Antibody (C-term)

Gene Names
NUAK1; ARK5
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARK5; Polyclonal Antibody; ARK5 Antibody (C-term); NUAK family SNF1-like kinase 1; AMPK-related protein kinase 5; Omphalocele kinase 1; NUAK1; KIAA0537; OMPHK1; anti-NUAK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-634 amino acids from the C-terminal region of human ARK5.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
604-634
Sequence Length
208
Applicable Applications for anti-NUAK1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-ARK5 Pab in mouse brain tissue lysate. ARK5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB) (Western blot analysis of anti-ARK5 Pab in mouse brain tissue lysate. ARK5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-NUAK1 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-NUAK1 antibody
References
Suzuki, A., et al., Mol. Cell. Biol. 24(8):3526-3535 (2004).
Suzuki, A., et al., Oncogene 22(40):6177-6182 (2003).
Suzuki, A., et al., J. Biol. Chem. 278(1):48-53 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74305
NCBI Official Full Name
NUAK family SNF1-like kinase 1
NCBI Official Synonym Full Names
NUAK family, SNF1-like kinase, 1
NCBI Official Symbol
NUAK1
NCBI Official Synonym Symbols
ARK5
NCBI Protein Information
NUAK family SNF1-like kinase 1
UniProt Protein Name
NUAK family SNF1-like kinase 1
UniProt Gene Name
NUAK1
UniProt Synonym Gene Names
ARK5; KIAA0537; OMPHK1; ARK5
UniProt Entry Name
NUAK1_HUMAN

Uniprot Description

NuaK1: a protein kinase of the CAMKL family. Phosphorylated and activated by AKT1 during glucose starvation. May activate ATM kinase under conditions of nutrient starvation. Suppresses apoptosis induced by nutrient starvation and death receptors via inhibition of caspase 8 activation.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; CAMKL family; NuaK subfamily

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: microtubule cytoskeleton; cytoplasm; nucleolus; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; metal ion binding; ATP binding

Biological Process: regulation of cell adhesion; cell adhesion; response to DNA damage stimulus; protein amino acid phosphorylation; regulation of cell proliferation

Research Articles on NUAK1

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Product Notes

The NUAK1 nuak1 (Catalog #AAA9203649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 604-634. The ARK5 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ARK5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the NUAK1 nuak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARK5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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