Rabbit eIF3B Polyclonal Antibody | anti-EIF3B antibody

eIF3B Rabbit pAb

Cat. Num. AAA4751934

• Gene Names: EIF3B; PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: eIF3B; Polyclonal Antibody; eIF3B Rabbit pAb; Eukaryotic translation initiation factor 3 subunit B; PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116; anti-EIF3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-EIF3B antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000-1:10,000; ICC/IF: 1:20-1:50; FC/FACS: 1:20; IP: 1:20-1:50
• Immunogen: A synthetic peptide of human eIF3B
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of eIF3B in Raji,COS7,3T3,Hela cell lysates using eIF3B Rabbit pAb(1:1000 diluted). Predicted band size:93KDa. Observed band size:117KDa.)

Related Product Information for anti-EIF3B antibody

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

NCBI and Uniprot Product Information

• NCBI GI #: 218512094
• NCBI GeneID: 8662
• UniProt Accession #: P55884; Q2NL77; Q9UMF9; A4D208
• Molecular Weight: 99,029 Da
• NCBI Official Full Name: Eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001}
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3, subunit B
• NCBI Official Symbol: EIF3B
• NCBI Official Synonym Symbols: PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001}; hPrt1; eIF-3-eta; eIF3 p110; eIF3 p116; prt1 homolog; eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3, subunit 9 eta,
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit B
• UniProt Gene Name: EIF3B
• UniProt Synonym Gene Names: eIF3b
• UniProt Entry Name: EIF3B_HUMAN

Uniprot Description

eIF3-eta: eukaryotic translation initiation factor 3 subunit 9. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: eukaryotic translation initiation factor 3 complex; nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; translation initiation factor binding; translation initiation factor activity; nucleotide binding; protein complex scaffold

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation