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Western Blot (WB) (Western blot analysis of eIF3B expression in (1) A431 cell lysate; (2) 293T cell lysate.)

Rabbit eIF3B Polyclonal Antibody | anti-eIF3B antibody

eIF3B Rabbit pAb

Gene Names
EIF3B; PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
eIF3B; Polyclonal Antibody; eIF3B Rabbit pAb; eIF3b; EIF3S9; hPrt1; PRT1; anti-eIF3B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
eIF3B Antibody detects endogenous levels of total eIF3B
Purity/Purification
Affinity purified
Form/Format
Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-eIF3B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50
Immunogen
A synthesized peptide derived from human eIF3B
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at +4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of eIF3B expression in (1) A431 cell lysate; (2) 293T cell lysate.)

Western Blot (WB) (Western blot analysis of eIF3B expression in (1) A431 cell lysate; (2) 293T cell lysate.)
Related Product Information for anti-eIF3B antibody
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
Product Categories/Family for anti-eIF3B antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
99,029 Da
NCBI Official Full Name
Eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001}
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3, subunit B
NCBI Official Symbol
EIF3B
NCBI Official Synonym Symbols
PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit B {ECO:0000255|HAMAP-Rule:MF_03001}; hPrt1; eIF-3-eta; eIF3 p110; eIF3 p116; prt1 homolog; eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3, subunit 9 eta,
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit B
UniProt Gene Name
EIF3B
UniProt Synonym Gene Names
eIF3b
UniProt Entry Name
EIF3B_HUMAN

Uniprot Description

eIF3-eta: eukaryotic translation initiation factor 3 subunit 9. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 7p22.3

Cellular Component: eukaryotic translation initiation factor 3 complex; nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; translation initiation factor binding; translation initiation factor activity; nucleotide binding; protein complex scaffold

Biological Process: cellular protein metabolic process; translation; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation

Research Articles on eIF3B

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Product Notes

The eIF3B eif3b (Catalog #AAA4757516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The eIF3B Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eIF3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 ICC/IF: 1:50-1:200 IP: 1:50 FC/FACS: 1:50. Researchers should empirically determine the suitability of the eIF3B eif3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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