Western Blot (WB)
(Western blot analysis of extracts of HeLa and Jurkat cells, using Phospho-eIF2alpha-S51 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Rabbit anti-Human eIF2alpha-S51 Polyclonal Antibody | anti-eIF2alpha-S51 antibody
Phospho-eIF2alpha-S51 pAb
IP: 1:50 - 1:100
Western Blot (WB)
(Western blot analysis of extracts of HeLa and Jurkat cells, using Phospho-eIF2alpha-S51 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa and Jurkat cells, using Phospho-eIF2alpha-S51 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by CA using 2.5ug Phospho-eIF2alpha-S51 antibody. Western blot was performed from the immunoprecipitate using Phospho-eIF2alpha-S51 antibody at a dilition of 1:1000.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells treated by CA using 2.5ug Phospho-eIF2alpha-S51 antibody. Western blot was performed from the immunoprecipitate using Phospho-eIF2alpha-S51 antibody at a dilition of 1:1000.)
NCBI and Uniprot Product Information
Observed: 38kDa
NCBI Description
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Uniprot Description
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Research Articles on eIF2alpha-S51
Similar Products
Product Notes
The eIF2alpha-S51 eif2s1 (Catalog #AAA9135611) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-eIF2alpha-S51 pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eIF2alpha-S51 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1000 - 1:2000 IP: 1:50 - 1:100. Researchers should empirically determine the suitability of the eIF2alpha-S51 eif2s1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SELSR. It is sometimes possible for the material contained within the vial of "eIF2alpha-S51, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
