Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PP6 Polyclonal Antibody: Human Testis PP6 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.)
Rabbit PP6C Polyclonal Antibody | anti-PP6C antibody
PP6C (protein phosphatase 6, catalytic subunit, FLJ92648, MGC12249, PP6C, PPP6, Serine/threonine-protein phosphatase 6 catalytic subunit) (HRP)
WB: 1:500-1:1000
IP: 10-20ug/ml
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PP6 Polyclonal Antibody: Human Testis PP6 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.)
Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PP6 Polyclonal Antibody: Human Testis PP6 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.)
Western Blot (WB)
(Western Blot Analysis: Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-6V (V/C) (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-6V (V/C) (~33kD).)
Western Blot (WB)
(Western Blot Analysis: Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-6V (V/C) (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-6V (V/C) (~33kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. Jurkat lysate (Lane1), Huvec lysate (Lane 2), PC12 lysate (Lane 3) and L6 lysate (Lane 4) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-6V (V/C) (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-6V (V/C) (~33kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. Jurkat lysate (Lane1), Huvec lysate (Lane 2), PC12 lysate (Lane 3) and L6 lysate (Lane 4) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-6V (V/C) (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-6V (V/C) (~33kD).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. Splice variants encoding different protein isoforms exist. The pseudogene of this gene is located on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
PPP6C: a Ser/Thr phosphatase. A functional homologue of budding yeast Sit4p and fission yeast ppe1, which are involved in cell cycle regulation.
Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 9q33.3
Cellular Component: cytosol
Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity
Biological Process: protein amino acid dephosphorylation; G1/S transition of mitotic cell cycle
Research Articles on PP6C
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Product Notes
The PP6C ppp6c (Catalog #AAA6495613) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP6C (protein phosphatase 6, catalytic subunit, FLJ92648, MGC12249, PP6C, PPP6, Serine/threonine-protein phosphatase 6 catalytic subunit) (HRP) reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's PP6C can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunoprecipitation (IP). IHC-P: 1:100 WB: 1:500-1:1000 IP: 10-20ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PP6C ppp6c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP6C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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