Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-4: Rat lung tissue,HT-29 cell,Mouse adrenal gland tissue and K-562 cell Primary antibody: 1/450 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)
Rabbit anti-Human SPCS2 Polyclonal Antibody | anti-SPCS2 antibody
SPCS2 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
SPCS2; Polyclonal Antibody; SPCS2 Antibody; KIAA0102; MGC117366; Signal peptidase complex subunit 2; anti-SPCS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total SPCS2 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.7 mg/ml (varies by lot)
Sequence Length
226
Applicable Applications for anti-SPCS2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:25-1:100
Immunohistochemistry: 1:25-1:100
Immunogen
Fusion protein of human SPCS2
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-4: Rat lung tissue,HT-29 cell,Mouse adrenal gland tissue and K-562 cell Primary antibody: 1/450 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)
Western Blot (WB)
(Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-4: Rat lung tissue,HT-29 cell,Mouse adrenal gland tissue and K-562 cell Primary antibody: 1/450 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/35,)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/35,)
Related Product Information for anti-SPCS2 antibody
SPCS2 (signal peptidase complex subunit 2), also known as SPC25 or microsomal signal peptidase 25 kDa subunit, is a 226 amino acid multi-pass membrane protein that localizes to both the microsome and the endoplasmic reticulum (ER), and belongs to the SPCS (signal peptidase complex subunit) family. Existing as a component of the microsomal signal peptidase complex which consists of five members, SPCS2 removes signal peptides from nascent proteins as they are translocated into the lumen of the ER. The gene encoding SPCS2 is located on human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome.
Product Categories/Family for anti-SPCS2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,003 Da
NCBI Official Full Name
signal peptidase complex subunit 2
NCBI Official Synonym Full Names
signal peptidase complex subunit 2
NCBI Official Symbol
SPCS2
NCBI Protein Information
signal peptidase complex subunit 2
UniProt Protein Name
Signal peptidase complex subunit 2
Protein Family
UniProt Gene Name
SPCS2
UniProt Synonym Gene Names
KIAA0102; SPC25; SPase 25 kDa subunit
UniProt Entry Name
SPCS2_HUMAN
Uniprot Description
SPCS2: Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Belongs to the SPCS2 family.
Protein type: EC 3.4.-.-; Membrane protein, integral; Membrane protein, multi-pass; Protease
Chromosomal Location of Human Ortholog: 11q13.4
Cellular Component: endoplasmic reticulum membrane; signal peptidase complex
Molecular Function: peptidase activity
Biological Process: protein targeting to ER; signal peptide processing
Similar Products
Product Notes
The SPCS2 spcs2 (Catalog #AAA9415413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPCS2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SPCS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the SPCS2 spcs2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPCS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
