Rabbit anti-Human Phospho-eIF2alpha (S51) Polyclonal Antibody | anti-eIF2alpha antibody

Phospho-eIF2alpha (S51) Polyclonal Antibody

Cat. Num. AAA2566964

• Gene Names: EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: Phospho-eIF2alpha (S51); Polyclonal Antibody; Phospho-eIF2alpha (S51) Polyclonal Antibody; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1; anti-eIF2alpha antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-eIF2alpha antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A phospho specific peptide corresponding to residues surrounding S51 of human EIF2S1
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells using Phospho-eIF2alpha (S51) Polyclonal Antibody.)

Related Product Information for anti-eIF2alpha antibody

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.

Product Categories/Family for anti-eIF2alpha antibody

Cancer; Epigenetics and Nuclear Signaling; Phosphorylated antibody

NCBI and Uniprot Product Information

• NCBI GI #: 4758256
• NCBI GeneID: 1965
• NCBI Accession #: NP_004085.1
• NCBI GenBank Nucleotide #: NM_004094.4
• UniProt Accession #: P05198
• Molecular Weight: 38 kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 2 subunit 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• NCBI Official Symbol: EIF2S1
• NCBI Official Synonym Symbols: EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• NCBI Protein Information: eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha
• UniProt Protein Name: Eukaryotic translation initiation factor 2 subunit 1
• UniProt Gene Name: EIF2S1
• UniProt Synonym Gene Names: EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
• UniProt Entry Name: IF2A_HUMAN

NCBI Description

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Uniprot Description

eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: RNA-binding; Translation; Translation initiation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; cytosol; nucleus; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression