Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using EHBP1 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit EHBP1 Polyclonal Antibody | anti-EHBP1 antibody
EHBP1 Antibody
Gene Names
EHBP1; HPC12; NACSIN
Reactivity
Human, Mouse, RatPredicted: Horse, Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
EHBP1; Polyclonal Antibody; EHBP1 Antibody; EH domain binding protein 1; NACSIN; anti-EHBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Horse, Rabbit, Dog
Predicted: Horse, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
EHBP1 Antibody detects endogenous levels of EHBP1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1196
Applicable Applications for anti-EHBP1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human EHBP1.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Endosome
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using EHBP1 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using EHBP1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-EHBP1 antibody
Description: May play a role in actin reorganization. Links clathrin-mediated endocytosis to the actin cytoskeleton. May act as Rab effector protein and play a role in vesicle trafficking (PubMed:14676205, PubMed:27552051). Required for perinuclear sorting and insulin-regulated recycling of SLC2A4/GLUT4 in adipocytes (By similarity).
Post Translational Modifications: Prenylated (Probable). Farnelysation (predominant) and geranylgeranylation has been observed in vitro.
Subunit Structure: Interacts with EHD1 (By similarity). Interacts with EHD2. Interacts with RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively.
Similarity: The CAAX motif is a signal for prenylation and required for endosomal colocalization with Rab8 and Rab10. The bivalent Mical/EHBP Rab binding (bMERB) domain, mediates binding to Rab8, Rab10, Rab10, Rab13 and Rab15 (in their GTP-bound forms).
Post Translational Modifications: Prenylated (Probable). Farnelysation (predominant) and geranylgeranylation has been observed in vitro.
Subunit Structure: Interacts with EHD1 (By similarity). Interacts with EHD2. Interacts with RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); at least in case of RAB8A may bind 2 molecules of RAB8A simultaneously through a high and a low affinity binding site, respectively.
Similarity: The CAAX motif is a signal for prenylation and required for endosomal colocalization with Rab8 and Rab10. The bivalent Mical/EHBP Rab binding (bMERB) domain, mediates binding to Rab8, Rab10, Rab10, Rab13 and Rab15 (in their GTP-bound forms).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 140 kDa
Predicted: 141 kDa
Predicted: 141 kDa
NCBI Official Full Name
EH domain-binding protein 1 isoform 2
NCBI Official Synonym Full Names
EH domain binding protein 1
NCBI Official Symbol
EHBP1
NCBI Official Synonym Symbols
HPC12; NACSIN
NCBI Protein Information
EH domain-binding protein 1
UniProt Protein Name
EH domain-binding protein 1
Protein Family
UniProt Gene Name
EHBP1
UniProt Synonym Gene Names
KIAA0903; NACSIN
UniProt Entry Name
EHBP1_HUMAN
NCBI Description
This gene encodes an Eps15 homology domain binding protein. The encoded protein may play a role in endocytic trafficking. A single nucleotide polymorphism in this gene is associated with an aggressive form of prostate cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Research Articles on EHBP1
1. Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
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Product Notes
The EHBP1 ehbp1 (Catalog #AAA9611722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EHBP1 Antibody reacts with Human, Mouse, Rat Predicted: Horse, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's EHBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the EHBP1 ehbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EHBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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