Rat GDF15 Monoclonal Antibody | anti-GDF15 antibody

Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15

Cat. Num. AAA246403

• Gene Names: GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
• Reactivity: Chimpanzee, Macaque
• Applications: Immunohistochemistry, Immunohistochemistry, Western Blot, ELISA
• Purity: Protein G Purified chromatography
• Synonyms: GDF15; Monoclonal Antibody; Rat Monoclonal [clone 6D12.H10.E4] (IgG) to Human GDF15; Anti-GDF15 Antibody (clone 6D12.H10.E4) IHC-plus; MIC1; MIC-1; NAG-1; NRG-1; NSAID-regulated gene 1 protein; PLAB; NSAID-activated gene 1 protein; Placental TGF-beta; PTGFB; GDF-15; PTGF-beta; Human GDF15; anti-GDF15 antibody

• Host: Rat
• Reactivity: Chimpanzee, Macaque
• Clonality: Monoclonal
• Isotype: IgG2a,I
• Clone Number: 6D12.H10.E4
• Specificity: This antibody reacts with the C-terminus of endogenous NAG-1 protein from human tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
• Purity/Purification: Protein G Purified chromatography
• Form/Format: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 308

• Applicable Applications for anti-GDF15 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB), ELISA
• Application Notes: IHC-Paraffin (10 ug/ml); Western Blot (1:1000); ELISA: (1:200); ; Optimal dilution to be determined by the researcher.; ; Usage Summary: This protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kD in size corresponding to NAG-1 monomer and dimer, respectively using the appropriate cell lysate or extract.
• Family/Subfamily: TGF beta/ not assigned-TGF beta
• Immunogen Species: GDF15 antibody raised against Mouse
• Antigen Type: Recombinant protein
• Immunogen: GDF15 antibody was raised against this Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E Coli corresponding to full-length mouse NAG-1 protein. Cross reactivity to MBP is negative.
• Epitope: C-Terminus
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable several weeks at 4°C as an undiluted liquid.

Immunohistochemistry (IHC)

(Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Product Categories/Family for anti-GDF15 antibody

Family: TGF beta

NCBI and Uniprot Product Information

• NCBI GI #: 153792495
• NCBI GeneID: 9518
• NCBI Accession #: NP_004855.2
• NCBI GenBank Nucleotide #: NM_004864.2
• UniProt Accession #: Q99988; O14629; P78360; Q9BWA0; Q9NRT0
• NCBI Official Full Name: growth/differentiation factor 15
• NCBI Official Synonym Full Names: growth differentiation factor 15
• NCBI Official Symbol: GDF15
• NCBI Official Synonym Symbols: PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
• NCBI Protein Information: growth/differentiation factor 15; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; prostate differentiation factor; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1
• UniProt Protein Name: Growth/differentiation factor 15
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF15
• UniProt Synonym Gene Names: MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1
• UniProt Entry Name: GDF15_HUMAN

NCBI Description

Bone morphogenetic proteins (e.g., BMP9; MIM 605120) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM, Oct 2009]

Uniprot Description

GDF15: disulfide-linked. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; cell-cell signaling; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; signal transduction; cell development