Rabbit EGR1 Polyclonal Antibody | anti-EGR1 antibody

RABBIT ANTI EGR1

Cat. Num. AAA224123

• Gene Names: EGR1; TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
• Reactivity: Bovine (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Rhesus Monkey (Expected from Sequence), Sheep (Expected from Sequence)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: EGR1; Polyclonal Antibody; RABBIT ANTI EGR1; anti-EGR1 antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chimpanzee (Expected from Sequence), Dog (Expected from Sequence), Rhesus Monkey (Expected from Sequence), Sheep (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognizes human Early Growth Response Protein 1, (EGR1), a 57.5 kDa nuclear protein belonging to the EGR family of C2H2-type zinc-finger proteins. ; ; EGR1 functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Research has also demonstrated that EGR1 expression is elevated in a range of cancers including prostate cancer where EGR1 has been shown to promote the growth of tumour cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 543

• Applicable Applications for anti-EGR1 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Minimum Dilution: 10ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.01% Sodium Azide (NaN3)
• Immunogen: A synthetic peptide sequence corresponding to amino acid 94 - 108 of human EGR1.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to Human EGR1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human lung small cell carcinoma with Rabbit anti Human EGR1 (MBS224123))

Related Product Information for anti-EGR1 antibody

MBS224123 recognizes human Early Growth Response Protein 1, (EGR1), a 57.5 kDa nuclear protein belonging to the EGR family of C2H2-type zinc-finger proteins. EGR1 functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Research has also demonstrated that EGR1 expression is elevated in a range of cancers including prostate cancer where EGR1 has been shown to promote the growth of tumour cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4503493
• NCBI GeneID: 1958
• NCBI Accession #: NP_001955.1
• NCBI GenBank Nucleotide #: NM_001964.2
• UniProt Accession #: P18146
• Molecular Weight: 57,507 Da
• NCBI Official Full Name: early growth response protein 1
• NCBI Official Synonym Full Names: early growth response 1
• NCBI Official Symbol: EGR1
• NCBI Official Synonym Symbols: TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268
• NCBI Protein Information: early growth response protein 1; EGR-1; nerve growth factor-induced protein A; transcription factor ETR103; transcription factor Zif268; zinc finger protein 225; zinc finger protein Krox-24
• UniProt Protein Name: Early growth response protein 1
• Protein Family: Early growth response protein
• UniProt Gene Name: EGR1
• UniProt Synonym Gene Names: KROX24; ZNF225; EGR-1; NGFI-A
• UniProt Entry Name: EGR1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014]

Uniprot Description

EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; regulation of long-term neuronal synaptic plasticity; response to nutrient levels; circadian rhythm; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; response to amphetamine; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; BMP signaling pathway; response to ethanol; learning and/or memory; cellular response to insulin stimulus; positive regulation of neuron apoptosis; response to glucose stimulus; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus; T cell differentiation; negative regulation of apoptosis; regulation of protein sumoylation