Western Blot (WB)
(EFCAB4B Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the EFCAB4B antibody detected the EFCAB4B protein (arrow).)
Rabbit anti-Human EFCAB4B Polyclonal Antibody | anti-EFCAB4B antibody
EFCAB4B, NT (EFCAB4B, CRACR2A, EF-hand calcium-binding domain-containing protein 4B, Calcium release-activated calcium channel regulator 2A) (FITC)
Gene Names
CRACR2A; EFCAB4B
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
EFCAB4B; Polyclonal Antibody; NT (EFCAB4B; CRACR2A; EF-hand calcium-binding domain-containing protein 4B; Calcium release-activated calcium channel regulator 2A) (FITC); Calcium release-activated calcium channel regulator 2A; anti-EFCAB4B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-EFCAB4B antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
EFCAB4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-74 amino acids from the N-terminal region of human EFCAB4B.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(EFCAB4B Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the EFCAB4B antibody detected the EFCAB4B protein (arrow).)
Western Blot (WB)
(EFCAB4B Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the EFCAB4B antibody detected the EFCAB4B protein (arrow).)
Related Product Information for anti-EFCAB4B antibody
Ca(2+)-binding protein that plays a key role in store-operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex.
Product Categories/Family for anti-EFCAB4B antibody
References
Chalasani, N., et al. Gastroenterology 139(5):1567-1576(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Srikanth, S., et al. Nat. Cell Biol. 12(5):436-446(2010). Aston, K.I., et al. J. Androl. 30(6):711-725(2009). Lim, J., et al. Cell 125(4):801-814(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,193 Da
NCBI Official Full Name
EF-hand calcium-binding domain-containing protein 4B isoform a
NCBI Official Synonym Full Names
calcium release activated channel regulator 2A
NCBI Official Symbol
CRACR2A
NCBI Official Synonym Symbols
EFCAB4B
NCBI Protein Information
EF-hand calcium-binding domain-containing protein 4B; CRAC regulator 2A; CRAC channel regulator 2A; EF-hand calcium binding domain 4B; calcium release-activated channel regulator 2A; calcium release-activated calcium channel regulator 2A; Ca2+ release-act
UniProt Protein Name
EF-hand calcium-binding domain-containing protein 4B
UniProt Gene Name
CRACR2A
UniProt Synonym Gene Names
EFCAB4B; CRAC channel regulator 2A
UniProt Entry Name
EFC4B_HUMAN
Uniprot Description
EFCAB4B: Ca(2+)-binding protein that plays a key role in store- operated Ca(2+) entry (SOCE) in T-cells by regulating CRAC channel activation. Acts as a cytoplasmic calcium-sensor that facilitates the clustering of ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca(2+) concentration. It thereby regulates CRAC channel activation, including translocation and clustering of ORAI1 and STIM1. Upon increase of cytoplasmic Ca(2+) resulting from opening of CRAC channels, dissociates from ORAI1 and STIM1, thereby destabilizing the ORAI1-STIM1 complex. Belongs to the EFCAB4 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 12p13.32
Cellular Component: Golgi apparatus; membrane; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; calcium ion binding
Biological Process: activation of store-operated calcium channel activity; immune system process; positive regulation of calcium ion transport
Research Articles on EFCAB4B
1. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
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Product Notes
The EFCAB4B cracr2a (Catalog #AAA6294503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EFCAB4B, NT (EFCAB4B, CRACR2A, EF-hand calcium-binding domain-containing protein 4B, Calcium release-activated calcium channel regulator 2A) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EFCAB4B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EFCAB4B cracr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EFCAB4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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