Mouse P-Cadherin ELISA Kit | CDH3 elisa kit

Mouse P-Cadherin PicoKine ELISA Kit

Cat. Num. AAA176527

• Gene Names: Cdh3; Cadp; Cdhp; Pcad; AI385538; P-cadherin
• Reactivity: Mouse
• Synonyms: P-Cadherin; Mouse P-Cadherin PicoKine ELISA Kit; Cadherin-3; cadherin 3; type 1; P-cadherin (placental); AI385538; CADH3_HUMAN; Cadherin 3; Cadherin 3 precursor; Cadherin 3 type 1; Cadp; Calcium dependent adhesion protein placental; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; P cadherin (placental); P-cadherin; PCAD; Placental cadherin; CDH3 elisa kit

• Reactivity: Mouse
• Specificity: Oligomeric forms of natural and recombinant mouse P-Cadherin
• Sequence Length: 822

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse P-Cadherin PicoKine ELISA Kit standard curve )

Related Product Information for CDH3 elisa kit

Principle of the assay: mouse P-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for P-Cadherin has been precoated onto 96-well plates. Standards(NSO,E100-G647) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse P-Cadherin amount of sample captured in plate.
Background: P-Cadherin, also known as Cadherin-3(CDH3), is a protein that in humans is encoded by the CDH3 gene. This gene is a classical cadherin from the cadherin superfamily. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. Cells expressing CDH3 also adhered to one another more tightly than the parental cell line.

NCBI and Uniprot Product Information

• NCBI GI #: 341940301
• NCBI GeneID: 12560
• UniProt Accession #: P10287; Q61465; Q8BSL6
• Molecular Weight: 90,612 Da
• NCBI Official Full Name: Cadherin-3
• NCBI Official Synonym Full Names: cadherin 3
• NCBI Official Symbol: Cdh3
• NCBI Official Synonym Symbols: Cadp; Cdhp; Pcad; AI385538; P-cadherin
• NCBI Protein Information: cadherin-3; placental cadherin; RPE-specific cadherin
• UniProt Protein Name: Cadherin-3
• UniProt Gene Name: Cdh3
• UniProt Synonym Gene Names: Cdhp; P-cadherin
• UniProt Entry Name: CADH3_MOUSE

NCBI Description

This gene encodes a calcium-dependent cell-cell adhesion protein containing five cadherin domains. The encoded protein plays a role in epithelial outgrowth, such as that which occurs during the development of hair follicles and limb buds. Loss of function of the related gene in humans results in ectodermal dysplasia, ectrodactyly, and macular dystrophy and congential hypotrichosis with juvenile macular dystrophy. This gene is located in the vicinity of similar cadherin genes on chromosome 8. The proprotein is further cleaved into a functional chain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

CDH3: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with CDCP1. Expressed in some normal epithelial tissues and in some carcinoma cell lines. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Cellular Component: cell-cell adherens junction; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: metal ion binding; calcium ion binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; negative regulation of transforming growth factor-beta2 production; hair cycle process; retinal homeostasis; cell-cell adhesion; positive regulation of monophenol monooxygenase activity; negative regulation of catagen; keratinization; positive regulation of melanin biosynthetic process; Wnt receptor signaling pathway through beta-catenin; homophilic cell adhesion; cell adhesion