Goat CYP24 / CYP24A1 Polyclonal Antibody | anti-CYP24 antibody

Goat Polyclonal to Human CYP24 / CYP24A1

Cat. Num. AAA248173

• Gene Names: CYP24A1; CP24; HCAI; CYP24; P450-CC24
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Horse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CYP24 / CYP24A1; Polyclonal Antibody; Goat Polyclonal to Human CYP24 / CYP24A1; Anti-CYP24 / CYP24A1 Antibody (Internal) IHC-plus; CYP24A1; 24-OHase; CP24; CYP24; Cytochrome P450-CC24; Exo-mitochondrial protein; HCAI; p450-CC24; Vitamin D 24-hydroxylase; Cytochrome P450 24A1; Vitamin D(3) 24-hydroxylase; Human CYP24; anti-CYP24 antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Human; Predicted Reactivity: Monkey, Horse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CYP24A1 / CYP24. This antibody is expected to recognize both reported isoforms (NP_000773.2; NP_001122387.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 514

• Applicable Applications for anti-CYP24 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kD band observed in Human and Rat Liver lysates (calculated MW of 51.2kD according to NP_001122387.1). Recommended concentration: 0.1-0.3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RKYDIQATDN from an internal region of human CYP24A1 / CYP24 (NP_000773.2; NP_001122387.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Elephant, Panda, Horse (90%); Rat, Dog, Bat, Xenopus (80%).
• Immunogen Type: Synthetic peptide
• Immunogen: CYP24 / CYP24A1 antibody was raised against synthetic peptide C-RKYDIQATDN from an internal region of human CYP24A1 / CYP24 (NP_000773.2; NP_001122387.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Elephant, Panda, Horse (90%); Rat, Dog, Bat, Xenopus (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-CYP24 / CYP24A1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(CYP24A1 antibody (0.1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-CYP24 antibody

Family: Cytochrome P450

NCBI and Uniprot Product Information

• NCBI GI #: 55770850
• NCBI GeneID: 1591
• NCBI Accession #: NP_000773.2
• NCBI GenBank Nucleotide #: NM_000782.4
• UniProt Accession #: Q07973; Q15807; Q32ML3; Q5I2W7
• Molecular Weight: 43,051 Da
• NCBI Official Full Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: cytochrome P450, family 24, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP24A1
• NCBI Official Synonym Symbols: CP24; HCAI; CYP24; P450-CC24
• NCBI Protein Information: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
• UniProt Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• UniProt Gene Name: CYP24A1
• UniProt Synonym Gene Names: CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase
• UniProt Entry Name: CP24A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; EC 1.14.13.126

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: mitochondrial inner membrane

Molecular Function: iron ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity

Biological Process: osteoblast differentiation; steroid metabolic process; vitamin metabolic process; response to vitamin D; xenobiotic metabolic process; vitamin D metabolic process; vitamin D catabolic process

Disease: Hypercalcemia, Infantile