EEF1A1 Polyclonal Antibody | anti-EEF1A1 antibody

EEF1A1 Polyclonal Antibody

Cat. Num. AAA126370

• Gene Names: EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: EEF1A1; Polyclonal Antibody; EEF1A1 Polyclonal Antibody; CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; FLJ25721; GRAF-1EF; HNGC:16303; LENG7; MGC102687; MGC131894; MGC16224; PTI1; eEF1A-1; anti-EEF1A1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 462

• Applicable Applications for anti-EEF1A1 antibody: WB, IF
• Application Notes: WB: 1:500 - 1:2000; IF: 1:20 - 1:100
• Source: Rabbit
• Buffer: PBS with 0.02% sodium azide, 50% glycerol,pH 7.3
• Immunogen: Recombinant protein of human EEF1A1
• Calculated Molecular Weight: 47kDa/50kDa
• Observed MW: 50kDa
• Preparation and Storage: Store at -20°C.; Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EEF1A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using EEF1A1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-EEF1A1 antibody

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3).

Product Categories/Family for anti-EEF1A1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 55584035
• NCBI GeneID: 1915
• UniProt Accession #: P68104; P04719; P04720; Q6IQ15
• NCBI Official Full Name: Elongation factor 1-alpha 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 alpha 1
• NCBI Official Symbol: EEF1A1
• NCBI Official Synonym Symbols: CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
• NCBI Protein Information: elongation factor 1-alpha 1; EF-1-alpha-1; EF1a-like protein; CTCL tumor antigen; elongation factor Tu; cervical cancer suppressor 3; elongation factor 1 alpha subunit; prostate tumor-inducing protein 1; eukaryotic elongation factor 1 A-1; leukocyte receptor cluster member 7; leukocyte receptor cluster (LRC) member 7; translation elongation factor 1 alpha 1-like 14; glucocorticoid receptor AF-1 specific elongation factor; eukaryotic translation elongation factor 1 alpha 1-like 14
• UniProt Protein Name: Elongation factor 1-alpha 1
• UniProt Gene Name: EEF1A1
• UniProt Synonym Gene Names: EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
• UniProt Entry Name: EF1A1_HUMAN

NCBI Description

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Protein type: Nucleolus; Translation; Translation elongation

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: extracellular space; cortical actin cytoskeleton; eukaryotic translation elongation factor 1 complex; membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity; tRNA binding

Biological Process: cellular protein metabolic process; translational elongation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; translation; gene expression