Rabbit Casein Kinase 2 Polyclonal Antibody | anti-CK2 antibody

Casein Kinase 2 (CK2) (MaxLight 750)

Cat. Num. AAA6467403

• Gene Names: CSNK2A1; CKII; CK2A1; CSNK2A3
• Reactivity: Human, Chicken, Frog, Mouse, Rabbit, Rat
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Casein Kinase 2; Polyclonal Antibody; Casein Kinase 2 (CK2) (MaxLight 750); anti-CK2 antibody

• Host: Rabbit
• Reactivity: Human, Chicken, Frog, Mouse, Rabbit, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human casein kinase 2 at 44kD and an unknown band at 66kD. Species Crossreactivity: Mouse. Predicted crossreactivity with rat, rabbit, chicken, frog and starfish.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-CK2 antibody: FLISA, Western Blot (WB)
• Application Notes: WB: 1-4ug/ml will detect Casein Kinase 2 in RIPA lysates from mouse 3T3 fibroblasts. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Casein Kinase 2 (4ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.; Not suitable for use in Immunoprecipitation.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide (C-SRARVYTDVNTHRPRE) corresponding to aa7-22 of human Casein Kinase 2, KLH-conjugated.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WB: 3T3 cell lysate was probed with C2085-60 (4ug/ml).)

Product Categories/Family for anti-CK2 antibody

Antibodies; Protein Kinases

References

1. Willert, K., et al, EMBO J. 16: 3089-3096, 1997. 2. Pinna, L.A. and F. Meggio, Prog. Cell Cycle Res. 3: 77-97, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 4503095
• NCBI GeneID: 1457
• NCBI Accession #: NP_001886.1
• NCBI GenBank Nucleotide #: NM_001895
• UniProt Accession #: P68400; P19138; P20426; Q14013; Q5U065; B4DYS6; D3DVV8; Q8NEV1
• Molecular Weight: 45,144 Da
• NCBI Official Full Name: casein kinase II subunit alpha isoform a
• NCBI Official Synonym Full Names: casein kinase 2, alpha 1 polypeptide
• NCBI Official Symbol: CSNK2A1
• NCBI Official Synonym Symbols: CKII; CK2A1; CSNK2A3
• NCBI Protein Information: casein kinase II subunit alpha; CK II alpha 3; protein kinase CK2; CK2 catalytic subunit alpha; casein kinase II alpha 1 subunit; casein kinase II alpha 1 polypeptide pseudogene
• UniProt Protein Name: Casein kinase II subunit alpha
• UniProt Gene Name: CSNK2A1
• UniProt Synonym Gene Names: CK2A1; CK II alpha
• UniProt Entry Name: CSK21_HUMAN

NCBI Description

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CK2A1: an ubiquitous protein kinase of the CK2 family. Exists as a tetramer composed of two catalytic subunits, alpha and alpha-prime, and two regulatory beta subunits. The beta subunits undergo autophosphorylation. The isoforms are rarely specified in publications. Two splice variant isoforms have been found. Participates in Wnt signaling. Phosphorylates E2 ubiquitin conjugating enzyme UBC3B inducing its interaction with beta-TRCp and enhancing beta-catenin degradation. May control IkB-alpha and p27Kip1 degradation. component of a CK2-SPT16-SSRP1 complex composed of SSRP1, PT16 CK2-A1, CK2-A2 and CK2-B the complex associating following UV irradiation. Interacts with RNPS1. Mouse transgene causes mammary gland hyperplasia and lymphoma, and activation by bovine parasites leads to fatal lymphoproliferation. Expression and activity are elevated in lung tumors and breast tumors. Antisense drives apoptosis of tumor cell lines and xenografts. Involved in DNA break repair by phosphorylation of scaffold protein XRCC1, phosphorylation of BRCA1, and phosphorylation of p53 in response to UV irradiation. Drosophila CK2 (Timekeeper) is involved in circadian regulation. Phosphorylates and binds to a major component of the inclusion bodies seen in Parkinson?s patients. Inhibitors: 4,5,6,7-tetrabromobenzotriazole (TBB).

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; CK2 family

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: Sin3 complex; plasma membrane; NuRD complex; PcG protein complex; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein N-terminus binding; Hsp90 protein binding; ATP binding

Biological Process: axon guidance; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; rhythmic process; positive regulation of protein catabolic process; negative regulation of caspase activity; mitotic cell cycle; signal transduction; positive regulation of cell growth; positive regulation of Wnt receptor signaling pathway; protein amino acid phosphorylation